Synergistic Protective Effects of Cordyceps militaris Polysaccharide Supplemented Yogurt on Alcoholic Liver Injury in Kunming Mice

被引：0
|
作者：
Han Y. [1 ]
Liu Y. [1 ]
Chen W. [1 ]
机构：
[1] Key Laboratory of Food Processing Technology and Quality Control in Universities of Shandong Province, School of Food Science and Engineering, Shandong Agricultural University, Tai'an
来源：
Shipin Kexue/Food Science | 2020年 / 41卷 / 01期
关键词：
Alcoholic liver injury; Cordyceps militaris polysaccharide; Synergistic protective effect; Yogurt;
D O I：
10.7506/spkx1002-6630-20181120-228
中图分类号：
学科分类号：
摘要：
Purpose: The protective effects of Cordyceps militaris polysaccharide supplemented probiotic yogurt on alcoholic liver injury in mice were investigated. Methods: Different doses (0.03%, 0.06%, 0.12%) of the probiotic yogurt following 40% ethanol were orally given to mice on a daily basis for 28 days. Body mass, liver index, serum and liver biochemical indicators were determined. Paraffin sections of liver tissue were prepared for observation of histopathological changes by hematoxylin and eosin staining. Results: In addition to remarkably improving liver damage, the probiotic yogurt at all doses could significantly reduce liver index and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities as well as total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels in serum, increase high-density lipoprotein cholesterol (HDL-C) levels in serum and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, and decrease malondialdehyde (MDA) level in liver tissue (P < 0.05). Compared with the model group, liver function of mice in the high-dose group was enhanced, and the serum levels of ALT and AST were decreased by 58.87% and 38.89%, respectively. Lipid metabolism in the liver was improved; TG, TC, LDL-C levels were decreased by 39.48%, 26.92% and 38.54%, respectively, and HDL-C level was increased by 20.67%. Liver antioxidant capacity was improved as reflected by the fact that the contents of GSH-Px and SOD in mouse liver were increased by 53.95% and 25.16%, respectively, and MDA content was reduced by 49.57%. Conclusion: The protective effect of probiotic yogurt supplemented with Cordyceps militaris polysaccharides on alcoholic liver injury in mice is significantly higher than that of plain yogurt, which is positively correlated with the concentration of Cordyceps militaris polysaccharides. Therefore, there is a synergistic protective effect of yoghurt and Cordyceps militaris polysaccharides on alcoholic liver injury in mice. © 2020, China Food Publishing Company. All right reserved.
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页码：209 / 214
页数：5
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共 16 条
  • [1] Reisa F.S., Barrosa L., Calhelha R.C., Et al., The methanolic extract of Cordyceps militaris (L) Link Fruiting Body Shows Antioxidant, Antibacterial, Antifungal and Antihuman Tumor Cell Lines properties, Food and Chemical Toxicology, 62, pp. 91-98, (2013)
  • [2] Ng T.B., Wang H.X., Pharmacological actions of Cordyceps, a prized folk medicine, The Journal of Pharmacy and Pharmacology, 57, pp. 1509-1519, (2005)
  • [3] Wang C., Chen Y., Hu M., Et al., In vitro antioxidant activities of the polysaccharides from Tricholoma lobayense, International Journal of Biological Macromolecules, 50, 3, pp. 534-539, (2012)
  • [4] Park D.K., Hayashi T., Park H.J., Arabinogalactan-type polysaccharides (APS) from Cordyceps militaris grown on germinated soybeans (GSC) induces innate immune activity of THP-1 monocytes through promoting their macrophage differentiation and macrophage activity, Food Science and Biotechnology, 21, 5, pp. 1501-1506, (2012)
  • [5] Sun Y., Liang H., Cai G., Et al., Sulfated modification of the water-soluble polysaccharides from Polyporus albicans mycelia and its potential biological activities, International Journal of Biological Macromolecules, 44, 1, pp. 14-17, (2009)
  • [6] Masarone M., Rosato V., Dallio M., Et al., Epidemiology and natural history of alcoholic liver disease, Reviews on Recent Clinical Trials, 11, 3, pp. 167-174, (2016)
  • [7] Song X., Liu Z., Zhang J., Et al., Antioxidative and hepatoprotective effects of enzymatic and acidic-hydrolysis of Pleurotus geesteranus mycelium polysaccharides on alcoholic liver diseases, Carbohydrate Polymers, 201, pp. 75-86, (2018)
  • [8] Afrin R., Arumugam S., Rahman A., Et al., Curcumin ameliorates liver damage and progression of NASH in NASH-HCC mouse model possibly by modulating HMGB1-NF-κB translocation, International Immunopharmacology, 44, pp. 174-182, (2017)
  • [9] Ohkawa H., Ohishi N., Yagi K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Analytical Biochemistry, 95, pp. 351-358, (1979)
  • [10] Zhang L., Zhao Q., Wang L., Et al., Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice, International Journal of Biological Macromolecules, 99, pp. 63-70, (2017)
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