Effect of Aged Wuyi Rock Tea on Relieving Dextran Sulfate Sodium-Induced Colitis and Regulating the Gut Microbiota in Mice

被引：1
|
作者：
Zeng H. [1 ]
Fang W. [1 ]
Zhou F. [1 ]
Tang J. [1 ]
Yue L. [1 ]
Ouyang J. [1 ]
Chen J. [1 ]
Wang Y. [1 ]
Huang J. [1 ]
Liu Z. [1 ]
机构：
[1] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-innovation Center of Education Ministry for Utilization of Botanical Functional Ingredien
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 13期
关键词：
aged Wuyi rock tea; colitis; dextran sulfate sodium; gut microbiota;
D O I：
10.7506/spkx1002-6630-20220309-117
中图分类号：
学科分类号：
摘要：
This research was performed in order to investigate the alleviative effect of aged Wuyi rock tea on dextran sulfate sodium (DSS)-induced colitis in mice. Fifty C57BL/6JGpt female mice were randomly and equally divided into five groups: control, DSS, DSS + infusion of 20-year-old Wuyi rock tea (DSS + OT01), DSS + infusion of 10-year-old Wuyi rock tea (DSS + OT11) and DSS + infusion of fresh Wuyi rock tea (DSS + OT20). The physiological and histopathological conditions of mice after Wuyi rock tea interventions, and the changes of serum inflammatory factors and cecal microbiota were analyzed. The results showed that aged Wuyi rock tea could significantly alleviate the symptoms of body mass loss, diarrhea, bloody stool, and colon length shortening, reduce inflammatory cell infiltration, and significantly inhibit the secretion of pro-inflammatory cytokines. In addition, aged Wuyi rock tea could alleviate the disorder of the gut microbiota, significantly down-regulate the relative abundance of Proteobacteria, Enterobacteriaceae and Escherichia, and up-regulate the relative abundance of Verrucomicrobia and Akkermansia. In summary, aged Wuyi rock tea can alleviate DSS-induced colitis in mice, and the tea produced in 2011 is more effective than that produced in 2001, which may be due to proper oxidation of catechins such as epigallocatechin gallate to produce thearubigins, with better anti-inflammatory and antioxidant properties. In addition, aged Wuyi rock tea is able to maintain intestinal homeostasis by regulating the relative abundance of Escherichia and Akkermansia in the intestine, which in turn alleviates the symptoms of DSS-induced colitis in mice such as body mass loss, diarrhea, bloody stool, colon length shortening, mucosal and crypt damage, inflammatory cell infiltration, and serum inflammatory factor overexpression. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：67 / 78
页数：11
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共 41 条
  • [1] UNGARO R, MEHANDRU S, ALLEN P B, Et al., Ulcerative colitis, Lancet, 389, pp. 1756-1770, (2017)
  • [2] BAUMGART D C, CARDING S R., Inflammatory bowel disease: cause and immunobiology, Lancet, 369, pp. 1627-1640, (2007)
  • [3] MOLODECKY N A, SOON I S, RABI D M, Et al., Increasing incidence and prevalence of the inflammatory bowel diseases with time, based on systematic review, Gastroenterology, 142, 1, pp. 46-54, (2012)
  • [4] HOU J K, EL-SERAG H, THIRUMURTHI S., Distribution and manifestations of inflammatory bowel disease in Asians, Hispanics, and African Americans: a systematic review, The American Journal of Gastroenterology, 104, 8, pp. 2100-2109, (2009)
  • [5] JAIRATH V, FEAGAN B G., Global burden of inflammatory bowel disease, Lancet Gastroenterology & Hepatology, 5, 1, pp. 2-3, (2020)
  • [6] HUANG Y, YANG Q, MI X, Et al., Ripened Pu-erh tea extract promotes gut microbiota resilience against dextran sulfate sodium induced colitis, Journal of Agricultural and Food Chemistry, 69, 7, pp. 2190-2203, (2021)
  • [7] WIRTZ S, POPP V, KINDERMANN M, Et al., Chemically induced mouse models of acute and chronic intestinal inflammation, Nature Protocols, 12, 7, pp. 1295-1309, (2017)
  • [8] LIU S, ZHAO W, LAN P, Et al., The microbiome in inflammatory bowel diseases: from pathogenesis to therapy, Protein & Cell, 12, 5, pp. 331-345, (2020)
  • [9] GEVERS D, KUGATHASAN S, DENSON L A, Et al., The treatment-naive microbiome in new-onset Crohn’s disease, Cell Host & Microbe, 15, 3, pp. 382-392, (2014)
  • [10] LLOYD-PRICE J, ARZE C, ANANTHAKRISHNAN A N, Et al., Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases, Nature, 569, pp. 655-662, (2019)
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