Experimental study on melanoma cell ablation by high-voltage nanosecond pulsed electric field

被引：2
|
作者：
Ma Z.-H. [1 ]
Liu Z. [1 ]
Yin S.-Y. [2 ]
Ma R.-W. [1 ]
Yan K.-P. [1 ]
机构：
[1] Institute of Industrial Ecology and Environment, Zhejiang University, Hangzhou
[2] The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou
来源：
Zhejiang Daxue Xuebao (Gongxue Ban)/Journal of Zhejiang University (Engineering Science) | 2021年 / 55卷 / 06期
关键词：
B16 tumor cell; Cell viability; Exponential pulse; Nanosecond pulsed electric field (nsPEF); Spark switch; Transmission line transformer (TLT); Tumor ablation;
D O I：
10.3785/j.issn.1008-973X.2021.06.018
中图分类号：
学科分类号：
摘要：
A repetitive high-voltage nanosecond pulsed electric field (RnsPEF) generation system was independently developed based on the spark switch and transmission line transformer (TLT) technology in order to analyze the key impact parameters of the process of malignant tumors ablation by high-voltage nanosecond pulsed electric field (nsPEF). The system can stably generate nanosecond exponential pulse. The experimental results proved the effectivity and controllability of RnsPEF on tumor cells ablation. B16 melanoma cells adherently seeded in six-well plates as the research object to analyze the effects of pulse number, peak voltage, repetition frequency and electrode spacing on tumor cells ablation. Cell counting kit-8 (CCK-8) was applied to measure cell viability of B16 tumor cells suspension in the cuvette after treated by pulses. The experimental results show that the pulsed electric field intensity and injected energy density of the applied RnsPEF play the key roles in determining the ablation effect. The repetition frequency hardly affects the ablation results. The pulsed electric field intensity threshold of RnsPEF ablating B16 melanoma cells is 6.8 kV/cm, and the injected energy density threshold is 11.4 J/cm3, as well as the optimal pulse number is 500 pulses. Copyright ©2021 Journal of Zhejiang University (Engineering Science). All rights reserved.
引用
收藏
页码：1168 / 1174and1198
相关论文
共 23 条
  • [1] BRAY F, FERLAY J, SOERJOMATARAM I, Et al., Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, CA: A Cancer Journal for Clinicians, 68, 6, pp. 394-424, (2018)
  • [2] WEAVER J C., Electroporation of cells and tissues, IEEE Transactions on Plasma Science, 28, 1, pp. 24-33, (2000)
  • [3] RUBINSKY B., Irreversible electroporation in medicine, Technology in Cancer Research and Treatment, 6, 4, pp. 255-260, (2016)
  • [4] ESSER A, SMITH K, GOWRISHANKAR T, Et al., Towards solid tumor treatment by irreversible electroporation: intrinsic redistribution of fields and currents in tissue, Technology in Cancer Research and Treatment, 6, 4, pp. 261-274, (2007)
  • [5] MIKLAVCIC D, SERSA G, BRECELJ E, Et al., Electrochemotherapy: technological advancements for efficient electroporation-based treatment of internal tumors, Medical and Biological Engineering and Computing, 50, 12, pp. 1213-1225, (2012)
  • [6] HABERL S, MIKLAVCIC D, SERSA G, Et al., Cell membrane electroporation-Part 2: the applications, IEEE Electrical Insulation Magazine, 29, 1, pp. 29-37, (2013)
  • [7] ZAGER Y, KAIN D, LANDA N, Et al., Optimization of irreversible electroporation protocols for in-vivo myocardial decellularization, Plos One, 11, 11, (2016)
  • [8] VROOMEN L G P H, PETRE E N, CORNELIS F H, Et al., Irreversible electroporation and thermal ablation of tumors in the liver, lung, kidney and bone: what are the differences?, Diagnostic and Interventional Imaging, 98, 9, pp. 609-617, (2017)
  • [9] SILK M T, WIMMER T, LEE K S, Et al., Percutaneous ablation of peribiliary tumors with irreversible electroporation, Journal of Vascular and Interventional Radiology, 25, 1, pp. 112-118, (2014)
  • [10] MARTIN R C G, KWON D, CHALIKONDA S, Et al., Treatment of 200 locally advanced (stage Ⅲ) pancreatic adenocarcinoma patients with irreversible electroporation, Annals of Surgery, 262, 3, pp. 486-494, (2015)
123