Effect of High-fat Diet on Cecal Intestinal Microbiota of Different Genders of Sprague-Dawley Rats Revealed by Miseq Sequencing

被引：0
|
作者：
Xia X. [1 ]
Li G. [2 ]
Liao L. [1 ]
Zeng L. [1 ]
Zheng J. [1 ,3 ]
Kan J. [1 ,3 ]
机构：
[1] College of Food Science, Southwest University, Chongqing
[2] College of Biotechnology, Southwest University, Chongqing
[3] Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing
来源：
Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 01期
关键词：
Cecum; Different genders; High-fat diet; High-throughput sequencing; Microbiota;
D O I：
10.16429/j.1009-7848.2019.01.029
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To investigate the effects of high-fat diet(HFD) on the cecal microbial community of different gender rats, Illumina Miseq sequencing approach was used to analysis the changes of cecal microbial community of HFD and normal control (NC) diet Sprague-Dawlay rats. After HFD, the perirenal fat and its coefficients of both male and female rats were significantly increased (P<0.05). Bacteroidetes and Firmicutes were the two most dominant phyla, accounting for >82% of the reads in all samples. Furthermore, the relative abundances of Firmicutes of female rats were higher than male rats. After HFD, the relative abundances of Firmicutes in both male and female rats were decreased, while those of Bacteroidetes were increased. Subsequently, at the genus level in both male and female rats, the relative abundances of Prevotella, unclassified Ruminococcaceae, Blautia, Bacteroides and unclassified Porphyromonadaceae were decreased, while those of incertae sedis and Alloprevotella were increased. Additionally, the relative abundances of unclassified Prevotellaceae, Phascolarctobacterium and unclassified Lachnospiraceae were decreased in HFD female rats, however, increased in HFD male rats. Thus, there were some differences between the effects of high-fat-diet on cecal intestinal microbiota of different gender rats. The results from this study may provide a basis for mechanism study of cecal intestinal microbiota participate in lipid metabolism of different gender rats. © 2019, Editorial Office of Journal of CIFST. All right reserved.
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页码：223 / 232
页数：9
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