Optimization of Ultrasonic-Enzyme-Assisted Deep Eutectic Solvents Extraction Process of Total Flavonoids from Mulberry Leaves and Its Antioxidant Activity

被引：0

作者：

Wu J. ^{[1
]}

Yang B. ^{[1
]}

Wang M. ^{[1
]}

Zhao P. ^{[1
]}

Ma J. ^{[1
]}

Huang Y. ^{[1
]}

Huang C. ^{[1
]}

机构：

[1] Chongqing Sericulture Science and Technology Research Institute, Chongqing

[2] Army Logistics University of PLA, Chongqing

来源：

Science and Technology of Food Industry | 2024年 / 45卷 / 03期

关键词：

antioxidant activity; deep eutectic solvent; response surface methodology; total flavonoids in mulberry leaves; ultrasonic-enzyme-assisted;

D O I：

10.13386/j.issn1002-0306.2023070033

中图分类号：

学科分类号：

摘要：

This study used ultrasound-enzyme-assisted deep eutectic solvents to establish a green and highly efficient total flavonoids extraction process from mulberry leaves. On the basis of the results of single-factor experiments, the extraction amount of total flavonoids was used as the response value, and the Box-Behnken response surface method was used to optimize, and the antioxidative ability of total flavonoids from mulberry leaves on DPPH radicals and ABTS+ radicals was studied. The results showed that when the choline chloride/fructose/alcohol molar ratio was 1:1:3, water content 30%, liquid-solid ratio 40 mL/g, ultrasonic power 360 W, ultrasonic temperature 40 ℃, ultrasonic time 40 min, dosage of enzyme 4%, the extraction amount of total flavonoids from mulberry leaves reached 46.58 mg/g. When the mass concentration of total flavonoids from mulberry leaves was 0.08 mg/mL, the scavenging rate of DPPH radicals was 98.36%. And when the mass concentration of total flavonoids from mulberry leaves was 0.2 mg/mL, the scavenging rate of ABTS+ radicals was 72.12%. Therefore, the ultrasonic-enzyme-assisted deep eutectic solvents method was green and simple to operate, and could effectively improve the extraction yield of total flavonoids from mulberry leaves. This method provided a scientific basis for the development and utilization of mulberry leaf resources. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.

引用

页码：31 / 39

页数：8

共 32 条

[1] CHEN Xing, Comparative analysis of alkaloids and phenol content in mulberry tree, (2021)
[2] ZHANG Qian, ZHANG Lihua, Chemical constituents and development and utilization of mulberry leaves[J], Hubei Agricultural Sciences, 59, 15, (2020)
[3] LU Tenghui, Ethanol extract from Morus alba L. leaves：Optimization of the extraction process and its application in chilled pork preservation, (2021)
[4] WEI Fangmei, Antioxidant and hypoglycemic activities of mulberry leaves extract, tea polyphenols and their compounds, (2019)
[5] Peng WEN, Tenggen HU, Linhardt J, Et al., Mulberry： A review of bioactive compounds and advanced processing technology[J], Trends in Food Science & Technology, 83, pp. 138-158, (2019)
[6] HUANG Jinzhi, SHI Xuping, HU Guiping, Et al., Evaluation on comprehensive functional quality and active ingredients of leaves from different mulberry varieties[J], Guizhou Agricultural Sciences, 50, 6, pp. 112-118, (2022)
[7] WU Qiguo, Yeqing HU, Systematic evaluation of the mechanisms of mulberry leaf （Morus alba L.） acting on diabetes based on network pharmacology and molecular docking[J], Combinatorial Chemistry & High Throughput Screening, 24, 5, pp. 668-682, (2021)
[8] BAI Huixin, JIANG Wei, WANG Xufang, Et al., Component changes of mulberry leaf tea processed with honey and its application to in vitro and in vivo models of diabetes[J], Food Additives & Contaminants: Part A, 38, 11, pp. 1840-1852, (2021)
[9] CHEN Yuan, NI Jiajia, Hongwei LI, Effect of green tea and mulberry leaf powders on the gut microbiota of chicken[J], BMC Veterinary Research, 15, 1, (2019)
[10] KWAK K W, HAN M S, KIM S Y, Et al., A role of mulberry leaves in improving resistance to virus ediated disease in Allomyrina dichotoma[J], Entomological Research, 50, 2, (2020)

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