Effects of Intraburst Frequency on Electroporation of A375 Melanoma Cells Induced by High-frequency Nanosecond Pulse Bursts

被引：0
|
作者：
Xu J. [1 ,2 ]
Mi Y. [1 ]
Wu X. [1 ]
Li J. [3 ]
Tang J. [3 ]
机构：
[1] State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing
[2] Huizhou Power Supply Bureau, Guangdong Power Grid Company Limited, Huizhou
[3] First Affiliated Hospital, Chongqing Medical Science University, Chongqing
来源：
Gaodianya Jishu/High Voltage Engineering | 2023年 / 49卷 / 12期
基金：
中国国家自然科学基金;
关键词：
A375 melanoma cells; cumulative effect; high-frequency nanosecond pulse bursts; intraburst frequency; irreversible electroporation;
D O I：
10.13336/j.1003-6520.hve.20220468
中图分类号：
学科分类号：
摘要：
High-frequency nanosecond pulse bursts can effectively kill tumor cells, whereas, the killing mechanism, especially the effect of the intraburst frequency on the induced electroporation, is still unclear. Taking A375 melanoma cells as the research object, we carried out in vitro cell experiments such as cell viability detection, short-term/long-term fluorescent dye detection, and cell micro-morphological detection to study the effect of the intraburst frequency of high-frequency nanosecond pulse bursts on cell membrane electroporation. The results of cell viability experiments show that high-frequency nanosecond pulse bursts can effectively kill cells, and the intraburst frequency has a threshold of 100 kHz that significantly reduces cell viability. The results of fluorescent dye experiments reveal that the decrease in cell viability is caused by irreversible electroporation, and there is a cumulative effect of intraburst frequency on electroporation. The results of scanning electron microscopy directly verify that the high-frequency nanosecond pulse bursts induce irreversible electroporation of tumor cells. The experimental results in this paper provide an experimental basis for the mechanism of high-frequency nanosecond pulse bursts killing tumor cells. © 2023 Science Press. All rights reserved.
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页码：5292 / 5301
页数：9
相关论文
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