Extraction of Astaxanthin by LCST-type Ionic Liquid-salt Aqueous Biphasic Systems

被引：0
|
作者：
Zhang L. [1 ]
Gao J. [1 ,2 ,3 ]
Wei Y. [1 ]
Nie F. [1 ]
Ji H. [1 ,2 ,3 ]
Liu S. [1 ,2 ,3 ]
Mao W. [1 ,2 ,3 ]
机构：
[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, Guangdong
[2] Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088,, Guangdong
[3] Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang, 524088, Guangdong
来源：
Journal of Chinese Institute of Food Science and Technology | 2020年 / 20卷 / 04期
关键词：
Aqueous biphasic system; Astaxanthin; Extraction; Haematococcus pluvialis; Ionic liquid;
D O I：
10.16429/j.1009-7848.2020.04.022
中图分类号：
学科分类号：
摘要：
Natural astaxanthin has outstanding biological activity and high economic value. However, many studies are still limited to the astaxanthin extraction with organic solvents such as acetone, which causes great pressure on the environment, and reduces the production safety. To explore an efficient and green process for astaxanthin extraction, novel ionic liquid (IL)-based aqueous biphasic systems (ABSs) were constructed. The effects of IL, temperature, pH on the phase behavior of the ABSs and astaxanthin distribution were investigated. The results showed that tributyloctylphosphonium bromide ([P4448]Br), tributyloctylphosphonium chlorine ([P4448]Cl), tetrabutylphosphonium bromide ([P4444]Br), tetrabutylphosphonium chloride [P4444]Cl, tetrabutylphosphonium trifluoroacetate ([P4444]CF3COO), and tetrabutylammonium bromide ([N4444]Br) could form low critical solution temperature (LCST)-type ABSs with K2HPO4/KH2PO4. [P4448]Br has the strongest ability to form ABS and the [P4448]Br-based ABS showed the highest extraction yield of astaxanthin. When the ultrasonic power of 75 W and ultrasonic time of 60 min, the extraction yield of astaxanthin in obtained from Haematococcus pluvialis by [P4448]Br-K2HPO4/KH2PO4 ABS was 96.09%. The results help us to understand the correlation between the phase behavior of the ABS and the extraction of biomolecule, and provide an important theoretical basis for the astaxanthin extraction from bioresources. © 2020, Editorial Office of Journal of CIFST. All right reserved.
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页码：170 / 178
页数：8
相关论文
共 39 条
  • [1] Zhao X.Y., Liu H.K., Zhang X.W., Et al., Astaxanthin from Haematococcus pluvialis microencapsulated by spray drying: Characterization and antioxidant activity, Journal of the American Oil Chemists Society, 96, 1, pp. 93-102, (2018)
  • [2] Guerin M., Huntley M.E., Olaizola M., Haematococcus astaxanthin: Applications for human health and nutrition, Trend in Biotechnology, 21, 5, pp. 210-216, (2003)
  • [3] Du X.P., Li B., Ying H., Et al., Inhibitory effect of astaxanthin on pancreatic lipase with inhibition kinetics integrating molecular docking simulation, Journal of Functional Foods, 48, pp. 551-557, (2018)
  • [4] Ambati R.R., Phang S.M., Ravi S., Et al., Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications-a review, Marine Drugs, 12, 1, pp. 128-152, (2014)
  • [5] Ranga R.A., Sindhuja H.N., Dharmesh S.M., Et al., Effective inhibition of skin cancer, tyrosinase, and antioxidative properties by astaxanthin and astaxanthin esters from the green alga Haematococcus pluvialis, Journal of Agricultural and Food Chemistry, 61, 16, pp. 3842-3851, (2013)
  • [6] Kornchanok W., Rueangyotchanthana K., Wayakanon P., Et al., The inhibition of Caco-2 proliferation by astaxanthin from Xanthophyllomyces dendrorhous, Journal of Medical Microbiology, 67, 4, pp. 507-513, (2018)
  • [7] Regnier P., Jorge B., Rodriguez-Ruiz V., Et al., Astaxanthin from Haematococcus pluvialis prevents oxidative stress on human endothelial cells without toxicity, Marine Drugs, 13, 5, pp. 2857-2874, (2015)
  • [8] Sun A.C., You K.O., Jiye L., Et al., High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium based ionic liquid/water mixtures, Bioresource Technology, 274, pp. 120-126, (2019)
  • [9] Antonio M., Juri R., Patrizia C., Et al., Extraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using generally recognized as safe solvents and accelerated extraction, Journal of Biotechnology, 283, pp. 51-61, (2018)
  • [10] Cheng X., Qi Z.B., Burdyny T., Et al., Low pressure supercritical CO<sub>2</sub> extraction of astaxanthin from Haematococcus pluvialis demonstrated on a microfluidic chip, Bioresource Technology, 250, pp. 481-485, (2018)
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