Optimization of Extraction Process of Total Saponins from Rubus crataegifolius Bunge. Root by Response Surface Methodology and Its Antioxidant Activity

被引：0
|
作者：
Yang X. [1 ]
Bai Z. [1 ]
Xu Y. [1 ]
Liang B. [1 ]
Zhou H. [1 ]
机构：
[1] College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin
来源：
Science and Technology of Food Industry | 2021年 / 42卷 / 18期
关键词：
extraction technology; in vitro antioxidant activity; response surface methodology; roots; Rubus crataegifolius Bunge; total saponins;
D O I：
10.13386/j.issn1002-0306.2020120266
中图分类号：
学科分类号：
摘要：
In this study, the extraction process of total saponins from Rubus crataegifolius Bunge. roots was investigated and optimized by response surface method. The effects of ethanol concentration, extraction time and solid-liquid ratio on the extraction rate of total saponins from pallets were investigated. The results showed that the optimal extraction conditions of the total saponins from the pallets were: extraction time 2 h, ethanol concentration 71%, liquid-solid ratio 28:1 mL/g, the predicted value was 3.49%. Under these conditions, the extraction rate of total saponins from R. crataegiolius Bunge. roots was 3.366%. The IC50 (mg/mL) values of total saponins extract from R. crataegifolius Bunge. roots against DPPH·, ABTS+· and superoxide anion free radicals were 0.0782, 0.277 and 0.9954 mg/mL, respectively. Therefore, the response surface method to optimize the extraction process of total saponins from R. crataegifolius Bunge. roots was effective, reliable, stable and feasible, and this article would provide scientific basis for the development and utilization of total saponins from pallets as natural antioxidants. © 2021 Editorial Department of Science and Technology of Food Science. All rights reserved.
引用
收藏
页码：183 / 189
页数：6
相关论文
共 35 条
  • [1] Zhou Y., Changbai mountain plant resources in China, (2010)
  • [2] Wei Z B, Sun J M, Li P F, Et al., Antioxidant activity constituents from root of Rubus crataegifolius[J], China Journal of Chinese Materia Medica, 44, 3, pp. 1-7, (2018)
  • [3] Ni W, Zhang X, Bi H, Et al., Preparation of a glucan from the roots of Rubus crataegifolius Bge. and its immunological activity[J], Carbohydrate Research, 344, pp. 2512-2518, (2009)
  • [4] Zhao W, Li C, Liu L M, Et al., Chemical constituents from the roots of Rubus crataegifolius Bge.[J], Chinese Pharmaceutical Journal, 52, 3, pp. 193-195, (2017)
  • [5] Zhang P, Xiao X Y, Zhang N P, Et al., Review on research of the chemical constituents and pharmacological activities of plants of Rosa-ceae[J], Chinese Pharmaceutical Affairs, 22, 8, pp. 721-726, (2008)
  • [6] Zhang X, Fang J B, Liang Z Y., Purification and initial studies of the water-soluble polysaccharide RCP from the roots of Rubus crataegiflolius Bge.[J], Special Wild Economic Animal and Plant Research, 1, pp. 40-42, (2005)
  • [7] Lee J H, Ham Y A, Choi S H, Et al., Activity of crude extract of Rubus crataegifolius roots as a potent apoptosis inducer and DNA topoisomerase I inhibitor[J], Archives of Pharmacal Research, 23, 4, pp. 338-343, (2000)
  • [8] Jung S W, Shin M H, Jung J H, Et al., A triterpene glucosyl ester from the roots of Rubus crataegifolius[J], Archives of Pharmacal Research, 24, 5, pp. 412-415, (2001)
  • [9] Li Q Y., Study of chemical constituent and hepatoprotective activity of Rubus crataegifolius Bunge, (2013)
  • [10] Wen L W, Zhang Q L, Qing G L, Et al., Research advances on biological activities and application of saponins[J], Animal Husbandry and Feed Science, 41, 4, pp. 90-96, (2020)
1234