Tea Seed Saponin Combined with Aerobic Exercise Ameliorated Lipid Metabolism and Oxidative Stress in High-Fat Diet-Fed Mice

被引：0
|
作者：
Cao W. [1 ,2 ]
Liu S. [1 ]
Li J. [1 ,3 ]
Qin H. [1 ]
Su Y. [1 ]
Liang C. [1 ,4 ]
Li W. [1 ,5 ]
Zeng Z. [1 ,6 ]
Song J. [1 ,7 ,8 ,9 ]
机构：
[1] School of Public Health, Guilin Medical University, Guilin
[2] School of Public Health, Zhejiang Chinese Medical University, Hangzhou
[3] School of Public Health, Xuzhou Medical University, Xuzhou
[4] School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan
[5] Department of Clinical Nutrition, Liuzhou People’s Hospital, Liuzhou
[6] Xiangya School of Public Health, Central South University, Changsha
[7] Guangxi Health Commission Key Laboratory of Entire Lifecycle Health and Care, Guilin Medical University, Guilin
[8] Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Health, Guilin Medical University, Guilin
[9] Department of Clinical Nutrition, the Second Hospital Affiliated to Guilin Medical University, Guilin
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 11期
关键词：
aerobic exercise; high-fat diet; lipid metabolism; oxidative stress; tea seed saponin;
D O I：
10.7506/spkx1002-6630-20220722-255
中图分类号：
学科分类号：
摘要：
Objective: The effect of tea seed saponin (TSS) combined with aerobic exercise (AE) on lipid metabolism and oxidative stress in mice on a high-fat diet (HFD) was investigated. Methods: Mice were randomly divided into a normal group and a model group. The normal group was fed on a maintenance diet and the model group on the HFD to establish an obesity model. After successful modeling, the mice were further divided into an HFD group, an HFD + TSS group, an HFD + AE group and an HFD + TSS-AE group. The levels of serum lipid metabolism and oxidative stress were determined by commercial kits. Hepatic steatosis was observed by hematoxylin-eosin (HE) staining. The protein levels of adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK), phosphorylated AMPK (p-AMPK), silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor γ coactlvator 1α (PGC-1α) and peroxisome proliferators-activated receptors γ (PPAR-γ) were detected by Western blot. Results: TSS combined with AE treatment significantly reduced body mass and subcutaneous, epididymal, perirenal and abdominal fat indexes in HFD mice (P < 0.05) and improved blood lipid disorders. The serum levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) were, respectively, reduced by 27.80%, 75.15%, 78.36% in the HFD + TSS + AE group compared with the HFD group. Hepatic steatosis was significantly improved in the HFD + TSS, HFD + AE and HFD + TSS-AE groups in comparison with the HFD group. The expression level of hepatic p-AMPK was increased by 64.19%, 2.12 times and 2.64 times, SIRT1 by 47.01%, 1.48% and 67.02%, PGC-1α by 2.03 times, 62.93% and 2.10 times, and PPAR-γ in skeletal muscle by 64.08%, 2.12 times and 3.34 times, respectively. After treated by TSS and AE, the serum and liver levels of superoxide dismutase (SOD) and glutathione (GSH) were increased in HFD mice, and serum malondialdehyde (MDA) levels were decreased. Conclusion: TSS combined with AE can reduce fat accumulation and improve lipid metabolism disorders and oxidative stress induced by HFD in mice. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：106 / 114
页数：8
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