Protective Effect of Corn Glycopeptide against Alcohol-induced LO2 Cells Injury

被引：0
|
作者：
Wang X. [1 ]
Tong L. [1 ]
Liu X. [1 ]
机构：
[1] Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology, College of Food and Biological Engineering, Qiqihar University, Heilongjiang, Qiqihar
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 01期
关键词：
alcohol metabolism enzymes; apoptosis; corn glycopeptide; LO2; cells; transglutaminase;
D O I：
10.16429/j.1009-7848.2023.01.002
中图分类号：
学科分类号：
摘要：
In the present work, the protective effect of corn glycopeptide intervention on alcohol-induced injury of LO2 cells was studied, and the possible mechanism of corn glycopeptide antagonizing alcoholic liver cell injury was investigated. The results show that compared with the alcohol model group, corn glycopeptide at 50 μg/mL remarkably increased the activities of alcohol metabolism enzymes (ADH and ALDH) and antioxidant enzymes (SOD, CAT and GSH-Px) as well as GSH content, decreased ROS and MDA levels in damaged LO2 cells. These results suggested that corn glycopeptide could enhance cytomembrane structural integrity by promoting alcohol metabolism and enhancing antioxidant activities, prevent the leakage of liver enzymes (AST, ALT and LDH) and reduce the percentage of early apoptosis and death of LO2 cells to achieve the effect of protecting the LO2 cells induced injury by alcohol. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：13 / 22
页数：9
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共 29 条
  • [1] Global status report on alcohol and health [EB/OL]
  • [2] ZHAO R J, HUO C Y, bIAN Y, Et al., Ultra - high -pressure processing improves proteolysis and release of bioactive peptides with activation activities on alcohol metabolic enzymes in vitro from mushroom foot protein, Food Chemistry, 231, pp. 25-32, (2017)
  • [3] LU C, XU W, ZHANG F, Et al., Ligustrazine prevents alcohol -induced liver injury by attenuating hepatic steatosis and oxidative stress, International Immunopharmacology, 29, pp. 613-621, (2015)
  • [4] DAI W O, CHEN C, FENG H T, Et al., Protection of Ficus pandurata Hance against acute alcohol-induced liver damage in mice via suppressing oxidative stress, inflammation, and apoptosis, Journal of Ethnopharmacology, 275, (2021)
  • [5] THAWLEY V., Acute liver injury and failure, Veterinary Clinics of North America -Small Animal Practice, 47, pp. 617-630, (2017)
  • [6] HWANG J M, TSENG T H, TSAI Y Y, Et al., Protective effects of baicalein on tert -butyl hydroperoxide-induced hepatic toxicity in rat hepatocytes, Journal of Biomedical Science, 12, pp. 389-397, (2005)
  • [7] BHANDARKAR M R, KHAN A., Antihepatotoxic effect of Nymphaea stellata willd., against carbon tetrachloride -induced hepatic damage in albino rats, Journal of Ethnopharmacology, 91, pp. 61-64, (2004)
  • [8] WU Y L, HUANG S H, HE C M, Et al., Dendrobium officinale flower extraction mitigates alcohol - induced liver injury in mice: Role of antisteatosis, antioxidative, and anti -inflammatory, Evidence - based Complementary and Alternative Medicine, 6, pp. 1-12, (2020)
  • [9] WANG X J, LIU X L, CONG W S, Et al., Effect of transglutaminase catalyzed glycosylation reaction on the biological activity of corn hexapeptide, China Brewing, 37, 3, pp. 78-83, (2018)
  • [10] WANG X J, LIU X L, ZHENG X b, Et al., Preparation of corn glycopeptides and evaluation of their antagonistic effects on alcohol -induced liver injury in rats, Journal of Functional Foods, 66, (2020)
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