Microsphere-based Fluorescence Immunochromatographic Assay for Quantitative Detection of Four Nitrofuran Metabolites in Aquatic Products

被引：0

作者：

Wu S. ^{[1
]}

Zhang B. ^{[1
]}

Zhang W. ^{[1
]}

Shao J. ^{[1
]}

Liu P. ^{[1
]}

Sheng W. ^{[1
]}

机构：

[1] State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin

来源：

Sheng, Wei (shengweijunchen@163.com) | 1600年 / Chinese Chamber of Commerce卷 / 42期

关键词：

Aquatic products; Fluorescent microsphere; Immunochromatographic assay; Nitrofuran; Quantitative detection;

D O I：

10.7506/spkx1002-6630-20201022-214

中图分类号：

学科分类号：

摘要：

An immunochromatographic assay (FMICA) using fluorescent microsphere-labeled antibody as the signal probe was developed for rapid quantitative detection of nitrofuran metabolite residues in aquatic products. The limits of detection (LOD) for NPAHD, NPAMOZ, NPAOZ, and NPSEM were 0.004, 0.01, 0.008 and 0.009 μg/L, and the linear ranges were 0.005-5, 0.01-10, 0.01-10, and 0.01-10 μg/L, respectively. The detection process took 10 min. The assay had good specificity. The spiked samples were detected by FMICA after derivatization treatment, and the recoveries of the four analytes ranged from 75.88% to 104.81%, with variation coefficients of less than 15%. Therefore, FMICA proved to be simple, fast, and low-cost and could be suitable for rapid quantitative detection of nitrofuran metabolites in aquatic products. © 2021, China Food Publishing Company. All right reserved.

引用

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页码：299 / 304

页数：5

共 19 条

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