Multi-walled carbon nanotubes enhanced electrochemical sensor for prothrombin time detection

被引：0
|
作者：
Li C. [1 ,2 ]
Guo Z. [1 ,2 ]
Zhang W. [1 ,2 ]
Yao J. [2 ,3 ]
Kong H. [1 ,2 ]
Yan R.-H. [4 ]
Zhou L.-Q. [1 ,2 ]
机构：
[1] University of Science and Technology of China, Hefei
[2] Key Laboratory of Biomedical Detection Technology, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou
[3] School of Electronic and Information Engineering, Soochow University, Suzhou
[4] Suzhou hospital Affiliated to Nanjing Medical University, Suzhou
来源：
Guangxue Jingmi Gongcheng/Optics and Precision Engineering | 2019年 / 27卷 / 06期
关键词：
Blood coagulation detection; Chronoamperometry; Electrochemical sensor; Multi-Walled Carbon Nanotubes(MWCNTs); Prothrombin Time(PT);
D O I：
10.3788/OPE.20192706.1345
中图分类号：
学科分类号：
摘要：
The determination of coagulation parameters is of crucial significance in the guided treatment of anticoagulant drug users and patients with liver diseases. Considering the challenges associated with commercial coagulation detection instruments such as high cost and complex operation, a MWCNT-enhanced screen-printed electrochemical sensor was developed for coagulation measurement. Initially, the measurement of thrombin substrate cleaved by thrombin was used to evaluate the feasibility of prothrombin time (PT) detection by chronoamperometry. Then the plasma PT parameters were measured and the results were validated using the SYSMEX CS 5100 optical coagulator. The response intensity of a MWCNT electrochemical sensor in thrombin validation experiments increase by (36±1)% compared with a general electrochemical sensor and the peak time coefficient of variation and peak current coefficients of variation are 2.99% and 3.27% respectively. The testing of PT values of different blood samples clearly shows discrimination. Three groups of blood samples are selected for repeated PT parameter measurements and the coefficients of variation are 2.26%, 3.22%, and 2.96% respectively. The linear fitting decision coefficient R2 is 0.986 for clinical results. The MWCNT screen-printed electrochemical sensor for PT testing has good repeatability and consistency, is easy to mass produce, reduces the cost of coagulation measurement, and is suitable for measurement in many circumstances. As such, this sensor has great potential in the field of point-of-care testing. © 2019, Science Press. All right reserved.
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页码：1345 / 1353
页数：8
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