A pathogen imprinted hybrid polymer capacitive sensor for selective Escherichia coli detection

被引:13
|
作者
Mugo, Samuel M. [1 ]
Lu, Weihao [1 ]
Dhanjai, Dhanjai [1 ,2 ]
机构
[1] Physical Sciences Department, MacEwan University, Edmonton,AB, Canada
[2] Department of Mathematical and Physical Sciences, Concordia University of Edmonton, Edmonton,AB, Canada
来源
Medical Devices and Sensors | 2020年 / 3卷 / 02期
基金
加拿大自然科学与工程研究理事会;
关键词
Carbon films - Electrochemical sensors - Pathogens - Capacitive sensors - Multiwalled carbon nanotubes (MWCN);
D O I
10.1002/mds3.10071
中图分类号
学科分类号
摘要
A versatile pathogen imprinted polymer electrochemical sensor has been developed for Escherichia coli detection. The sensor is based on layer by layer assembly of multi-walled carbon nanotubes (CNT), nanocellulose (CNC) films, integrated with polyaniline (PANI) doped phenylboronic acid (PBA). The sensing layer is a poly(methacrylic acid) based pathogen microcontact imprinted polymer (PIP). The PIP@PBA/PANI@CNT/CNC sensor exhibits a high affinity towards E. coli in real biological matrices, while effective in discriminating E. coli amidst other bacteria. Using capacitance and impedance as transduction methods, the PIP sensor recorded a low limit of E. coli detection of 8.7 ± 0.5 cfu/ml, with a rapid response of ≤5 min. © 2020 Wiley Periodicals, Inc.
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