Microwave Preparation of Common Lophatherum Herb Carbon Quantum Dots and Application in Cell Imaging

被引：0
|
作者：
Hu M.-Y. [1 ,2 ,3 ,4 ]
Liu K. [1 ,2 ,3 ,4 ]
Gao S.-Y. [1 ,2 ,3 ,4 ]
Sun T.-Y. [1 ,2 ,3 ,4 ]
Shi J.-C. [1 ]
Xu C.-Y. [1 ,2 ,3 ]
Xu L. [1 ,2 ,3 ]
机构：
[1] College of Materials Science and Engineering, Nanjing Forestry University, Nanjing
[2] Jiangsu Co-innovation Centre of Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing
[3] Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing Forestry University, Nanjing
[4] 2019 Jiangsu Graduate Workstation: Jingjiang Guolin Wood Industry Co., Ltd. (Workstation No. 2019_099), Jingjiang
来源：
Faguang Xuebao/Chinese Journal of Luminescence | 2022年 / 43卷 / 12期
基金：
中国国家自然科学基金;
关键词：
carbon quantum dots; cell imaging; common lophatherum herb; microwave method; response surface method;
D O I：
10.37188/CJL.20220256
中图分类号：
学科分类号：
摘要：
In this study, response surface methodology(RSM) was used to study the effects of microwave action time(T), microwave power(W) and the ratio of common lophatherum herb to deionized water(R) on the fluorescence quantum yield(QY) of N/Si-CQDs prepared by microwave method. The significance order of the influence of the above process parameters on QY, and the corresponding QY regression model and the best process parameters are obtained, and the optimization results are proved to be reliable through validation experiments. The average particle size of N/Si-CQDs obtained by the best process is small and evenly distributed, with good dispersion in water, excitation dependent emission characteristics, high fluorescence stability, low cytotoxicity to HEK293 cells. And the N/Si CQDs can be absorbed by cells to illuminate cells, so as to clearly distinguish cytoplasm and nucleus, which shows that the N/Si-CQDs can be used for cell imaging. This study not only provides a new idea for the high-value utilization of bamboo leaves, but also has reference value for improving the efficiency of microwave preparation of biomass carbon quantum dots and promoting their application in biomedical fields such as cell imaging. © 2022 Chines Academy of Sciences. All rights reserved.
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页码：2001 / 2013
页数：12
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