Screening， Adsorption Characteristics and Mechanism of Lactic Acid Bacteria with High Heterocyclic Aromatic Amines Adsorption Capacity

被引：0
|
作者：
Wang H. [1 ]
Liu P. [3 ]
Xiao Y. [2 ]
Su L. [1 ]
Jin Y. [1 ]
Zhao L. [1 ]
机构：
[1] Inner Mongolia Agricultural University, Hohhot
[2] Hohhot Sampling Monitoring and Major Activities Support Center, Hohhot
[3] Ulanqab Market Supervision and Administration, Ulanqab
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 06期
关键词：
adsorption; heterocyclic aromatic amines; lactic acid bacteria; screening;
D O I：
10.16429/j.1009-7848.2023.06.005
中图分类号：
学科分类号：
摘要：
To screen the lactic acid bactic with high PhIP adsorption capacity and to study adsorption characteristics by HPLC. The adsorption mechanism was discussed by scanning electron microscopy and Fourier transform infrared spectroscopy. The results showed that lactic acid bacteria 37X-6, 37X-15 and X3-1B had high adsorption capacity for PhIP, the adsorption rates were 80.43%, 83.36% and 76.69%, respectively. And the strains had the best adsorption effect when the cell concentration of strains was 1010 CFU/mL, pH was 6, temperature was 37C or adsorption time was 1.5 h. By heat treatment and enzyme treatment, it was found that the bacterial adsorption was a physical adsorption unrelated to its activity, and the bacterial adsorption rate increased after enzyme treatment. By scanning electron microscopy and Fourier infrared spectroscopy, it was found that PhIP cell surface was obviously damaged when strain adsorbed PhIP. The adsorption site of PhIP was mainly in the cell wall, and C-N, O-H, N-H, C-O groups were involved in the adsorption process. Lactic acid bacteria can adsorb PhIP, which was a physical adsorption, and its adsorption was related to the bacterial cell wall. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：41 / 47
页数：6
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