Mechanism of Agaricus blazei Polypeptide on Aging Mice Induced by D-Galactose

被引：0
|
作者：
Feng Q. [1 ,2 ]
Sun Y. [1 ]
Sun J. [2 ]
Yuan G. [2 ]
An L. [2 ]
机构：
[1] Food and Drug Engineering College, Jilin Province Economic Management Cadre College, Changchun
[2] School of Pharmacy, Beihua University, Jilin
来源：
Journal of Food Science and Technology (China) | 2023年 / 41卷 / 05期
关键词：
Agaricus blazei polypeptide; aging; D-galactose; Keap1; Nrf2; P53; transcriptome sequencing;
D O I：
10.12301/spxb202300229
中图分类号：
学科分类号：
摘要：
Polypeptides are important active substances in Agaricus blazei. To investigate the anti-aging effect of Agaricus blazei polypeptide (ABp), ABp was prepared by hydrolyzing Agaricus blazei protein with pepsin, and purified by column chromatography using a protein purification system. A mouse aging model was established by injecting D-galactose. The effects of ABp on aging model mice were investigated by behavioral experiments, serum biochemical indicators and transcriptome sequencing. Furthermore, Western blotting was used to explore the potential mechanism of ABp. The results indicated that the molecular weight of ABp was 3郾 3 kDa after separation and purification. ABp could significantly inhibit the elevated levels of reactive oxygen species and malondialdehyde in mouse serum caused by D-galactose, significantly enhanced the total antioxidant capacity and catalase activity in mouse serum. The transcriptome sequencing results showed significant differences in the expression levels of 295 genes in mouse hippocampus between ABp group and model group. Compared with the aging model group, ABp upregulated 79 genes and downregulated 216 genes in mouse hippocampus. The results of Western blotting indicated that the potential mechanism of ABp anti-aging might be related to the Keap1 / Nrf2 / P53 signaling pathway. This study aims to provide a theoretical basis for the development of anti-aging functional food of Agaricus blazei. © 2023 Beijing Technology and Business University, Department of Science and Technology. All rights reserved.
引用
收藏
页码：58 / 67
页数：9
相关论文
共 20 条
  • [1] LIU X, LUO Q, RAKARIYATHAM K, Et al., Antioxida-tion and anti-ageing activities of different stereoisomeric astaxanthin in vitro and in vivo, Journal of Functional Foods, 25, pp. 50-61, (2016)
  • [2] FENG Y, YU Y H, WANG S T, Et al., Chlorogenic acid protects D-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice, Pharmaceutical Biology, 54, 6, pp. 132-135, (2016)
  • [3] XU J, LI Y, REGENSTEIN J, Et al., In vitro, and, in vivo, anti-oxidation and anti-fatigue effect of monkfish liver hydrolysate, Food Bioscience, 18, pp. 9-14, (2017)
  • [4] TIAN B, WU Y, SHENG D, Et al., Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus ra-diodurans, Luminescence, 19, 2, pp. 78-84, (2004)
  • [5] DING J F, LI Y Y, XU J J, Et al., Study on effect of jel-lyfish collagen hydrolysate on anti-fatigue and anti-oxida-tion, Food Hydrocolloids, 25, 5, pp. 1350-1353, (2011)
  • [6] XU J, LI Y, REGENSTEIN J, Et al., In vitro and in vivo anti-oxidation and anti-fatigue effect of monkfish liver hy-drolysate, Food Bioscience, 18, pp. 9-14, (2017)
  • [7] CHANG J B, WU M F, YANG Y Y, Et al., Carbon tetra-chloride-induced hepatotoxicity and its amelioration by Agaricus blazei Murrill extract in a mouse model, In Vivo, 25, 6, pp. 971-976, (2011)
  • [8] JIANG L, YU Z, LIN Y, Et al., Low-molecular-weight polysaccharides from Agaricus blazei Murrill modulate the Th1 response in cancer immunity, Oncology Letters, 15, 3, pp. 3429-3436, (2018)
  • [9] REN C, ZHOU Y, XIAN S, Et al., Study on anti-infec-tion effect of polysaccharide from Agaricus blazei Murrill on mice, Agricultural Biotechnology, 7, 4, pp. 110-112, (2018)
  • [10] DA S D S A, CORREA V G, DE A G G, Et al., Agari-cus blazei bioactive compounds and their effects on hu-man health: benefits and controversies [J], Current Pharmaceutical Design, 23, 11, pp. 67-81, (2017)
12