Effect of lignin extracted by lactic acid-based deep eutectic solvent from rice straw on cellulase hydrolysis efficiency

被引：0
|
作者：
Pu F. [1 ]
Wu S. [1 ]
Zheng Y. [1 ]
Zheng Y. [1 ]
Hou X. [1 ]
机构：
[1] School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangdong, Guangzhou
来源：
Huagong Jinzhan/Chemical Industry and Engineering Progress | 2022年 / 41卷 / 09期
关键词：
adsorption; enzyme; inhibitory effect; lignin; lignocellulosic biomass; solvents;
D O I：
10.16085/j.issn.1000-6613.2021-2364
中图分类号：
学科分类号：
摘要：
Lactic acid-based DES has shown great capability in removal of lignin from biomass and conversion of lignocellulose to fuels, chemicals and materials. In this work, lignin was extracted by four DESs, in which lactic acid was used as hydrogen bond donor (HBD) and choline chloride, guanidine hydrochloride, arginine or betaine hydrochloride was used as the hydrogen bond acceptor (HBA), and its effect on the hydrolysis efficiency of Avicel was studied. The results showed that the adsorption capacity of lignin towards cellulase shows a good linear positive correlation with the hydrophobicity of lignin, and both of them are linearly negatively correlated with the enzymatic hydrolysis efficiency of Avicel. The nonspecific adsorption of lignin towards protein was the major cause of the inhibition, and the hydrophobic effect is mainly responsible for the adsorption of enzymes on lignin. Furthermore, the structural analysis of lignin revealed that the hydrogen bond interaction of aliphatic hydroxyl groups, phenolic hydroxyl groups, and the amino acids residues of proteins was the reason for promoting enzyme protein adsorption and reduced enzyme hydrolysis efficiency. Besides, the electrostatic repulsion generated by carboxyl group of lignin had weak effect on the hydrolysis efficiency of cellulase. DES with strong deconstruction severity may cause high hydroxyl group content, which always lead to strong inhibition of cellulase. Lactic acid/ choline chloride (LC) had the strongest inhibitory effect on enzyme due to its excellent lignin extraction and biomass deconstruction ability, followed by lactic acid guanidine hydrochloride and lactic acid betaine hydrochloride, and finally lactic acid arginine. This work is expected to improve the fundamental understanding of the knowledge of DES application in the field of biorefinery,and lay a foundation for the development of novel solvents-driven lignocellulosic biomass refining. © 2022 Chemical Industry Press. All rights reserved.
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页码：4937 / 4945
页数：8
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