Optimization of Purification Process of Polysaccharides from Dendrobium officinale by Response Surface Methodology

被引：0
|
作者：
Zhang X. [1 ]
Wei M. [1 ]
Yang Y. [1 ]
Huang Y. [1 ]
Su G. [1 ]
Liu G. [1 ]
Li F. [1 ]
Wu Z. [1 ]
Ding J. [1 ]
机构：
[1] School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou
来源：
Science and Technology of Food Industry | 2021年 / 42卷 / 16期
关键词：
aqueous two-phase extraction; Dendrobium officinale; polysaccharide; purification; response surface;
D O I：
10.13386/j.issn1002-0306.20201200261
中图分类号：
学科分类号：
摘要：
Objective: To optimize the purification process of crude polysaccharides from Dendrobium officinale by using response surface methodology. Methods: Through single factor experiments, the effects of (NH4)2SO4 concentration, C2H5OH concentration and extraction temperature on the extraction rate and protein removal rate of polysaccharides were investigated, and then a response surface test was performed. The response values of the extraction rate and protein removal rate of polysaccharides were used for statistical analysis. Results: The degree of influence of various factors on the polysaccharides extraction rate and protein removal rate in descending order was extraction temperature>(NH4)2SO4 concentration>C2H5OH concentration. Based on the total mass of the two-phase aqueous system, the optimal extraction conditions were as follows: 18.80% (NH4)2SO4, 25.00% C2H5OH, extraction temperature was 25 ℃, and the Dendrobium officinale polysaccharide extraction rate and protein removal rate were respectively 94.39% and 91.36%. Conclusion: This method could effectively purify the crude polysaccharides of Dendrobium officinale, and provided a theoretical basis for the development and utilization of Dendrobium officinale polysaccharides. © 2021 Jilin University Press. All rights reserved.
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页码：177 / 184
页数：7
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