Improved modeling strategy and result evaluation of steroid-induced femoral head necrosis in rabbits

被引：0
|
作者：
Yang F. [1 ]
Feng G. [2 ]
Li J. [2 ]
Niu X. [1 ]
机构：
[1] Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing
[2] Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing
来源：
Beijing Hangkong Hangtian Daxue Xuebao/Journal of Beijing University of Aeronautics and Astronautics | 2023年 / 49卷 / 07期
基金：
中国国家自然科学基金;
关键词：
animal model; femoral head necrosis; histological staining; Micro-CT; steroid;
D O I：
10.13700/j.bh.1001-5965.2021.0558
中图分类号：
学科分类号：
摘要：
The establishment of animal model of femoral head necrosis is of great significance for the clinical treatment of this disease. To successfully induce femoral head necrosis while reducing the mortality of modeling animals, this study improves the commonly used modeling method of steroid-induced femoral head necrosis in rabbits. Ninety male New Zealand white rabbits (2.8～3.2 kg) are randomly divided into control group, conventional model group and improved model group. The control group is only injected with normal saline; the conventional model group is injected with lipopolysaccharide and methylprednisolone; the improved model group is supplemented with penicillin, ranitidine, diclazuril, intestinal lubricant and lactasin based on conventional model. The results show that the death rate of the improved model group (13.3%) is much lower than that of the conventional model group (70%). The Micro-CT and histological results exhibit that the bone volume, bone volume/tissue volume ratio, bone mineral density and trabecular thickness in improved model group are significantly lower than those in control group, while the number of empty bone lacunae is significantly higher than that in control group. These results demonstrate the successful establishment of steroid-induced femoral head necrosis in rabbits. © 2023 Beijing University of Aeronautics and Astronautics (BUAA). All rights reserved.
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页码：1755 / 1760
页数：5
相关论文
共 18 条
  • [1] WANG C K, HO M L, WANG G J, Et al., Controlled-release of rhBMP-2 carriers in the regeneration of osteonecrotic bone, Biomaterials, 30, 25, pp. 4178-4186, (2009)
  • [2] MARUYAMA M, NABESHIMA A, PAN C C, Et al., The effects of a functionally-graded scaffold and bone marrow-derived mononuclear cells on steroid-induced femoral head osteonecrosis, Biomaterials, 187, pp. 39-46, (2018)
  • [3] ZHANG H X, ZHANG X P, XIAO G Y, Et al., In vitro and in vivo evaluation of calcium phosphate composite scaffolds containing BMP-VEGF loaded PLGA microspheres for the treatment of avascular necrosis of the femoral head, Materials Science and Engineering:C, 60, pp. 298-307, (2016)
  • [4] WAEWSAWANGWONG W, RUCHIWIT P, HUDDLESTON J I, Et al., Hip arthroplasty for treatment of advanced osteonecrosis: Comprehensive review of implant options, outcomes and complications, Orthopedic Research and Reviews, 8, pp. 13-29, (2016)
  • [5] STEVENS K, TAO C, LEE S U, Et al., Subchondral fractures in osteonecrosis of the femoral head: Comparison of radiography, CT, and MR imaging, American Journal of Roentgenology, 180, 2, pp. 363-368, (2003)
  • [6] QIN L, ZHANG G, SHENG H, Et al., Multiple bioimaging modalities in evaluation of an experimental osteonecrosis induced by a combination of lipopolysaccharide and methylprednisolone, Bone, 39, 4, pp. 863-871, (2006)
  • [7] SUN L, YUAN Y, NIU R, Et al., Comparative study on immunoreactions of small intestinal submucosa by irradiation and ethylene oxide sterilization treatments, Journal of Beijing University of Aeronautics and Astronautics, 46, 12, pp. 2245-2252, (2020)
  • [8] ZHANG L, SUN L, XU M H, Et al., Immunological response evaluation of absorbable collagen membrane in vivo, Journal of Beijing University of Aeronautics and Astronautics, 44, 4, pp. 879-886, (2018)
  • [9] XU J Z, GONG H P, LU S T, Et al., Animal models of steroid-induced osteonecrosis of the femoral head-a comprehensive research review up to 2018, International Orthopaedics, 42, 7, pp. 1729-1737, (2018)
  • [10] CHEN G R, WANG X F, WANG L M, Et al., Analysis on fetal factors and intervention of steriod-induced femoral head necrosis in rabbits, Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 22, 5, pp. 342-344, (2012)
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