Analysis of Changes in Volatile Components during Processing of Handmade Fuzhuan Tea

被引:4
|
作者
Li J. [1 ,2 ]
Xu Y. [1 ]
Chen M. [1 ]
Deng G. [1 ]
Wu K. [1 ]
Jiang L. [1 ]
机构
[1] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha
[2] School of Chemistry and Chemical Engineering, Zhaotong University, Zhaotong
来源
Shipin Kexue/Food Science | 2020年 / 41卷 / 24期
关键词
Aroma components; Fuzhuan tea; Gas chromatography-ion mobility spectroscopy; Headspace solid-phase microextraction-gas chromatographymass spectrometry;
D O I
10.7506/spkx1002-6630-20191226-316
中图分类号
学科分类号
摘要
Gas chromatography-ion mobility spectroscopy (GC-IMS) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) were used to analyze the changes in volatile components in Fuzhuan tea during fermentation and drying. The results of GC-IMS showed that there were 44 small-molecular volatile components (C3-C10) that differed between the two processing stages. The contents of volatile components increased continuously during the fermentation process. The contents of volatile components did not change significantly during the first four days of fermentation, but changed significantly by the eighth day, and then tended to change slowly later. During the drying process, the contents of volatile components increased gradually. Further, by HS-SPME-GC-MS, a total of 57 of C8-C15 organic compounds were identified, belonging to seven chemical classes, mainly terpenes, alcohols, aldehydes, esters, ketones and ethers. The contents and kinds of volatile components increased with fermentation and drying time. The contents and kinds of terpenes with characteristic wood-like and grassy odor decreased gradually, and the contents and kinds of alcohols, esters ketones and aldehydes with fruity, herbal and mushroom-like aroma as the characteristic odor increased. Finally, the odor of tea samples was characterized as mushroom-like and floral aromas integrating herbal, wood-like, flowery and fruity aroma notes. The results of GC-IMS and HS-SPME-GC-MS showed some differences from each other. Most of the components detected by GC-IMS were small molecules at low levels, while most of the components detected by HSSPME-GC-MS were large molecules at high levels. The general trend of changes in volatile components determined by HS-SPME-GC-MS was consistent with that determined by GC-IMS, and the change in odor-active volatile components was basically consistent with the sensory evaluation results. To sum up, combined use GC-IMS and HS-SPME-GC-MS can make up for each other's limitations and more comprehensively reflect changes in volatile components of samples. © 2020, China Food Publishing Company. All right reserved.
引用
收藏
页码:144 / 154
页数:10
相关论文
共 26 条
  • [1] VAUTZ W, FRANZKE J, ZAMPOLLI S, Et al., On the potential of ion mobility spectrometry coupled to GC pre-separation: a tutorial, Analytica Chimica Acta, 1024, pp. 52-64, (2018)
  • [2] GERHARDT N, BIRKENMEIER M, SANDERS D, Et al., Resolutionoptimized headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) for non-targeted olive oil profiling, Analytical and Bioanalytical Chemistry, 409, 16, pp. 3933-3942, (2017)
  • [3] HU X, WANG R R, GUO J J, Et al., Changes in the volatile components of candied kumquats in different processing methodologies with headspace-gas chromatography-ion mobility spectrometry, Molecules, 24, 17, pp. 354-361, (2019)
  • [4] CHEN T, QI X, CHEN M, Et al., Gas chromatography-ion mobility spectrometry detection of odor fingerprint as markers of rapeseed oil refined grade, Journal of Analytical Methods in Chemistry, 2019, pp. 1-8, (2019)
  • [5] CAVANNA D, ZANARDI S, DALL'ASTA C, Et al., Ion mobility spectrometry coupled to gas chromatography: a rapid tool to assess eggs freshness, Food Chemistry, 271, pp. 691-696, (2019)
  • [6] TIAN L L, ZENG Y Y, ZHENG X Q, Et al., Detection of peanut oil adulteration mixed with rapeseed oil using gas chromatography and gas chromatography-ion mobility spectrometry, Food Analytical Methods, 12, 10, pp. 2282-2292, (2019)
  • [7] ROUVROYE M D, WICAKSONO A, BOSCH S, Et al., Faecal scent as a novel non-invasive biomarker to discriminate between coeliac disease and refractory coeliac disease: a proof of principle study, Biosensors, 9, 2, pp. 130-141, (2019)
  • [8] BUNKOWSKI A, MADDULA S, DAVIES A N, Et al., One-year time series of investigations of analytes within human breath using ion mobility spectrometry, International Journal for Ion Mobility Spectrometry, 13, 3, pp. 141-148, (2010)
  • [9] KOCZULLA R, HATTESOHL A, SCHMID S, Et al., MCC/IMS as potential noninvasive technique in the diagnosis of patients with COPD with and without alpha 1-antitrypsin deficiency, International Journal for Ion Mobility Spectrometry, 14, 4, pp. 177-185, (2011)
  • [10] GALLEGOS J, ARCE C, JORDANO R, Et al., Target identification of volatile metabolites to allow the differentiation of lactic acid bacteria by gas chromatography-ion mobility spectrometry, Food Chemistry, 220, pp. 362-370, (2017)