Study on the denitrifying characteristics of oligotrophicaerobic denitrifying bacteria flora from source water reservoir

In order to reduce the nitrogen concentration in micro-polluted reservoirs, a mixed high efficient aerobic denitrifying flora-A1, dominated by Pseudomonas, was directional enrichment and domestication from the sediments of Lijiahe Reservoir (Xi'an). Shaker experiments showed that the nitrate nitrogen removal ratio of lean nutrient aerobic denitrifying flora A1reached 93.39% at 15h, and the average nitrate removal rate was 0.2073mg/ (L•h). The total nitrogen removal ratio was 52.11% and the average removal rate of total nitrogen was 0.1153mg/(L•h). There was no nitrite accumulation detected. Nitrogen balance analysis showed that about 45% of the initial nitrogen was removed and converted into gas products. Response surface method (RSM) results showed when C/N ratio=9.96, temperature=22.67℃, pH=8.01, speed=91r/min, dissolved oxygen=8.55mg/L, the optimum condition of total nitrogen (TN) removal could be obtained. © 2020, Editorial Board of China Environmental Science. All right reserved.