Establishment of a Method for the Identification of Plant-Derived Ingredients in Mānuka Honey

被引：0

作者：

Yang Y. ^{[1
,3
]}

Wang Y. ^{[1
]}

Liu M. ^{[1
]}

Niu N. ^{[2
]}

Huang W. ^{[1
]}

Wu Z. ^{[1
]}

Wang S. ^{[1
]}

Kang J. ^{[3
]}

Wu Y. ^{[1
]}

机构：

[1] Chinese Academy of Inspection and Quarantine, Beijing

[2] Food Inspection Center of Shenzhen Customs, Immigration and Quarantine, Shenzhen

[3] Shaanxi Institute of Product Quality Supervision and Inspection, Xi’an

来源：

Shipin Kexue/Food Science | 2023年 / 44卷 / 14期

关键词：

authentication; DNA; kānuka; mānuka honey; real-time polymerase chain reaction;

D O I：

10.7506/spkx1002-6630-20220726-290

中图分类号：

学科分类号：

摘要：

To establish a method to identify the authenticity of manuka honey, this study compared different methods of DNA extraction from mānuka honey pollen, and it developed a DNA extraction method for mānuka honey supernatant and a real-time polymerase chain reaction (real-time PCR) method for the detection of plant-derived ingredients in mānuka honey, including plant internal control, mānuka and kānuka. By analyzing the specificity, sensitivity, detection limit and comparing with the method of the New Zealand Ministry for Primary Industries (MPI), the feasibility, accuracy and equivalence of this method were confirmed. The proposed DNA detection method could replace the MPI method, not only shaking off the dependence on kits, but also making up for the deficiency of identifying mānuka honey pollen only. It has an important application value and scientific significance for the identification of mānuka honey. © 2023 Chinese Chamber of Commerce. All rights reserved.

引用

页码：352 / 359

页数：7

共 35 条

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