Structural and Functional Properties of Prolamin in Foxtail Millet before and after Cooking

被引:0
|
作者
Fu Y. [1 ]
Liu Z. [1 ]
Zhang F. [1 ]
Shen Q. [1 ]
机构
[1] College of Food Science & Nutritional Engineering, China Agricultural University, Key Laboratory of Plant Protein and Cereal Processing of Beijing, National Engineering Research Center for Fruits and Vegetables Processing, Beijing
来源
Shen, Qun (shenqun@cau.edu.cn) | 1600年 / Chinese Institute of Food Science and Technology卷 / 21期
关键词
Cooking; Foxtail millet; Functional properties; Prolamin; Structural properties;
D O I
10.16429/j.1009-7848.2021.11.012
中图分类号
学科分类号
摘要
Foxtail millet (FM) is usually eaten after cooking in daily life, however, studies about the structural and functional properties changes of the main storage protein prolamin in FM before and after cooking are limited. In this study, the prolamins from uncooked and cooked FM were extracted respectively, and the structural and functional changes of which were compared. Scanning electron microscopy results showed that the structure of prolamin from cooked FM was different from that from uncooked FM, as evidenced by occurrence of protein body cross-linking between each other and the changes in protein shape from circular to irregular. The differential scanning calorimetry results further confirmed that the denaturation temperature of prolamin in cooked FM was increased, thus demonstrating that the structure of which tended to be stable. In addition, the amino acid content of prolamin in cooked FM was significantly higher than that in uncooked foxtail millet. Moreover, the secondary structure analysis revealed that part of β-fold was converted to α-helix in prolamin from cooked FM, which indicated that its structure became stable. Furthermore, solubility of prolamin in cooked FM was decreased when compared with prolamin in uncooked FM. Taken together, significant structural and functional changes occurred in FM prolamin after cooking. © 2021, Editorial Office of Journal of CIFST. All right reserved.
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页码:96 / 104
页数:8
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