Redox-modulation, Suppression of “Oncogenic” Superoxide and Induction of Apoptosis in Burkitt’s Lymphoma Cells Using Geum urbanum L. Extracts

被引：1

作者：

Mileva M. ^{[1
]}

Dimitrova L. ^{[1
]}

Popova M. ^{[2
]}

Bankova V. ^{[2
]}

Krastev D. ^{[3
,4
]}

Najdenski H. ^{[1
]}

Zhelev Z. ^{[5
,6
]}

Aoki I. ^{[7
,8
]}

Bakalova-Zheleva R. ^{[7
,8
]}

机构：

[1] The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str.,Bl. 26, Sofia

[2] Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Akad. G. Bonchev Str.,Bl. 9, Sofia

[3] Medical University of Sofia, Medical Colleague “Jordanka Filaretova”, 3 Jordanka Filaretova Str., Sofia

[4] South-West University “Neofit Rilski”, 66 Ivan Mihailov Str., Blagoevgrad

[5] Medical Faculty, Trakia University, Students Town,Stara Zagora

[6] Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str.,Bl. 105, Sofia

[7] Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, Chiba

[8] Group of Quantum-state Controlled MRI, National Institute of Radiological Sciences (QST-NIRS), Chiba

来源：

International Journal Bioautomation | 2021年 / 25卷 / 04期

基金：

日本学术振兴会;

关键词：

Apoptosis; Burkitt’s lymphoma; Geum urbanum l; Reactive oxygen species; Total antioxidant capacity;

D O I：

10.7546/ijba.2021.25.4.000795

中图分类号：

学科分类号：

摘要：

Burkitt’s lymphoma is a highly aggressive type of non-Hodgkin’s lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in theinfected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate(EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial partson Burkitt's lymphoma cells (BLC), to elucidate their impact on oxidative stress andcell survival. BLC Raji was treated with EtOAc and n-BuOH extracts to analyze:cell viability; induction of apoptosis; hydroperoxides and reactive nitrogen species (RNS) by2’,7’-dichlorodihydrofluorescein assay; superoxide by dihydroethidium assay; totalantioxidant capacity by TAC assay. All extracts suppressed cell growth andinduce apoptosis. n-BuOH extracts possessed higher cytotoxicity and pro-apoptoticactivity compared to EtOAc. The fractions decreased the hydroperoxides and RNS levels.There was no correlation between the DCF fluorescence in the treated cells and theirviability (R = –0.3722; p > 0.05). Root extracts decreased the superoxide level, while theleaf extracts did not. There was a good correlation between the dihydroethidiumfluorescence in the treated cells and their viability (R = 0.9843; p < 0.01). All extractsincreased the TAC of BLC. G. urbanum extracts serve as redox-modulators andanti-inflammatory compounds, decreasing the intracellular level of “oncogenic” superoxideand cell proliferation © 2021 by the authors. Licensee Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)

引用

页码：315 / 330

页数：15

共 1 条

[1] The effect of Yarrowia lipolytica L-asparaginase on apoptosis induction and inhibition of growth in Burkitt's lymphoma Raji and acute lymphoblastic leukemia MOLT-4 cells
Mazloum-Ravasan, Sahand
Madadi, Elaheh
Fathi, Zahra
Mohammadi, Ali
Mosafer, Jafar
Mansoori, Behzad
Mokhtarzadeh, Ahad
Baradaran, Behzad
Darvishi, Farshad
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 146 : 193 - 201

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