Synthesis of (R)-epichlorohydrin catalyzed by cross-linked cell aggregates of epoxide hydrolase

被引：0

作者：

Zou S. ^{[1
]}

Jiang Z. ^{[1
]}

Wang Z. ^{[1
]}

Liu Z. ^{[1
]}

Zheng Y. ^{[1
]}

机构：

[1] National and Local Joint Engineering Research Center for Chiral Biomanufacturing, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang

来源：

Huagong Xuebao/CIESC Journal | 2020年 / 71卷 / 09期

关键词：

Biocatalysis; Chiral epichlorohydrin; Cross-linked cell aggregates; Epoxide hydrolase; Immobilization; Multiphase reaction;

D O I：

10.11949/0438-1157.20200447

中图分类号：

学科分类号：

摘要：

Polyethyleneimine (PEI) flocculation and glutaraldehyde (GA) crosslinking were used to prepare crosslinked cell aggregates (CLCAs) of whole cells of epoxide hydrolase. The effects of PEI concentration, GA concentration and diatomite carrier dosage on the activity recovery of CLCAs were investigated, the results show that the optimal values of PEI concentration, GA concentration and diatomite carrier dosage are 3% (vol), 1% (vol) and 6 g/L, respectively and activity recovery reached 88.4%. Using CLCAs as catalysts, racemic epichlorohydrin ((R, S)-ECH) as substrate, the synthesis of (R)-epichlorohydrin ((R)-ECH) in isooctane / phosphate buffer biphase system was investigated. The results show that under the condition of 3:7 volume ratio of isooctane to buffer, substrate concentration 800 mmol/L, addition of CLCAs 18 g/L, buffer pH 8.0, temperature 35℃, the molar yield of (R)-ECH reached 45.2%, with 99.1% ee. The operational stability of CLCAs in the two-phase system was investigated, and the viability of 9 batches reused remained basically unchanged, showing good operation stability. © 2020, Chemical Industry Press Co., Ltd. All right reserved.

引用

页码：4238 / 4245

页数：7

共 35 条

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