Monodispersed Au nanoparticles decorated MoS2 nanosheets with enhanced peroxidase-like activity based electrochemical H2O2 sensing for anticancer drug evaluations

Background: The unique size, physical and chemical properties, and ultra-high stability of nanozymes have attracted extensive attentions in sensing, but improvement of catalytic activity of the nanozymes is still an urgent issue. Given the ultra-high simulated enzyme activity of metal nanoparticles and the advantage of multi-enzyme catalysis, an Au-decorated MoS2 nanosheets (MoS2/Au NS) integrating the double peroxidase-like (POD) activity is developed. Results: By optimizing and adjusting the density of AuNPs, as well as its morphology and other parameters, a monodisperse and high-density distribution of AuNPs on MoS2 2 nanosheets was obtained, which can greatly improve the POD-like activity of MoS2/Au 2 /Au NS. Nafion solution was applied to assist the modification of MoS2/Au 2 /Au NS on the electrode surface so as to improved its stability. An electrochemical H2O2 2 O 2 detection platform was constructed by modifying MoS2/Au 2 /Au NS nanozyme on the SPCE using the conductive Nafion solution. And the negatively charged sulfonic acid group can eliminate negatively charged electroactive substances to improve the specificity. Then ascorbic acid was used to stimulate tumor cells to produce H2O2 2 O 2 as therapeutic model, an ultrasensitive chronocoulometry detection for H2O2 2 O 2 in cell lysate was established. The logarithmically of Delta Q and the logarithmically of H2O2 2 O 2 concentration showed a good linear relationship between 1 mu M and 500 mM, with a LOD value of 0.3 mu M. Significance: The developed H2O2 2 O 2 sensor has excellent stability, reproducibility (RSD = 2.3 %, n = 6) and selectivity, realized the quantitative detection of H2O2 2 O 2 in cell lysate. Compared with commercial fluorescence detection kits for H2O2 2 O 2 in cell lysate, it is worth mentioning that the electrochemical H2O2 2 O 2 sensor developed in this study is simpler and faster, with higher sensitivity and lower cost. This provides a potential substitute for disease diagnosis and treatment evaluation based on accurate detection of H2O2. 2 O 2 .