Oral food-derived whey protein isolate-Tremella Tremella fuciformis polysaccharides pickering emulsions with adhesive ability to delivery magnolol for targeted treatment of ulcerative colitis

被引：2

作者：

Zhang, Qian ^{[1
]}

Deng, Hongdan ^{[1
]}

Luo, Ruifeng ^{[1
,2
]}

Qi, Hu ^{[1
]}

Lei, Yicheng ^{[1
]}

Yang, Luping ^{[1
]}

Pang, Huiwen ^{[3
]}

Fu, Chaomei ^{[1
]}

Liu, Fang ^{[1
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Pharm Sch, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China

[2] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 999078, Macau, Peoples R China

[3] Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld 4072, Australia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 280卷

关键词：

Magnolol; Pickering emulsions; Whey protein isolate; Tremella fuciformis polysaccharides; Ulcerative colitis; INFLAMMATION; ACID;

D O I：

10.1016/j.ijbiomac.2024.135585

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Magnolol (Mag) is a promising natural compound with therapeutic potential for ulcerative colitis (UC). Here we designed and fabricated an oral food-grade whey protein isolate-Tremella fuciformis polysaccharides (WPI-TFPS) stabilized pickering emulsions to encapsulate Mag (Mag-WPI-TFPS) for targeted treatment of UC. With the assistance of the WPI-TFPS, pickering emulsions were well encapsulated and formed stable microparticles with a particle size of approximately 9.49 +/- 0.047 mu m, a 93.63 +/- 0.21 % encapsulation efficiency and a loading efficiency of 21.53 +/- 0.01 %. In vitro, the formulation exhibited sustained-release properties in simulated colon fluid with a cumulative release rate of 60.78 % at 48 h. In vivo, the Mag-WPI-TFPS specifically accumulated in the colon tissue for 24 h with stronger fluorescence intensity, which demonstrated that TFPS and WPI had a good adherence ability to inflamed mucosa by electrostatic attraction and ligand-receptor interactions. As expected, compared with Free-Mag, the oral administration of Mag-WPI-TFPS remarkably alleviated the symptoms of UC and protected the colon tissue in DSS-induced UC mice. More importantly, WPI-TFPS enhanced gut microbiota balance by increasing the diversity and relative abundances of Lactobacillaceae and Firmicutes. . Overall, this study presents a convenient, eco-friendly, food-derived oral formulation with potential as a dietary supplement for targeted UC treatment.

引用

页数：14