Design of Alginate/Gelatin Hydrogels for Biomedical Applications: Fine-Tuning Osteogenesis in Dental Pulp Stem Cells While Preserving Other Cell Behaviors

被引：5

作者：

Ferjaoui, Zied ^{[1
]}

Lopez-Munoz, Roberto ^{[2
]}

Akbari, Soheil ^{[3
]}

Chandad, Fatiha ^{[1
]}

Mantovani, Diego ^{[2
]}

Rouabhia, Mahmoud ^{[1
]}

D. Fanganiello, Roberto ^{[1
]}

机构：

[1] Univ Laval, Fac Medecine Dentaire, Oral Ecol Res Grp GREB, Quebec City, PQ G1V 0A6, Canada

[2] Laval Univ, Dept Min Met Mat Eng & Regenerat Med, Lab Biomat & Bioengn CRC Tier I, CHU Quebec, Quebec City, PQ G1V 0A6, Canada

[3] Univ Laval, Dept Genie Chim, Quebec City, PQ G1V 0A6, Canada

来源：

BIOMEDICINES | 2024年 / 12卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

alginate; gelatin; hydrogel; stiffness; DPSCs; osteogenic differentiation; SUBSTRATE STIFFNESS; MATRIX STIFFNESS; DIFFERENTIATION; PHOSPHATE; SCAFFOLD; INDUCTION; ADHESION;

D O I：

10.3390/biomedicines12071510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alginate/gelatin (Alg-Gel) hydrogels have been used experimentally, associated with mesenchymal stromal/stem cells (MSCs), to guide bone tissue formation. One of the main challenges for clinical application is optimizing Alg-Gel stiffness to guide osteogenesis. In this study, we investigated how Alg-Gel stiffness could modulate the dental pulp stem cell (DPSC) attachment, morphology, proliferation, and osteogenic differentiation, identifying the optimal conditions to uncouple osteogenesis from the other cell behaviors. An array of Alg-Gel hydrogels was prepared by casting different percentages of alginate and gelatin cross-linked with 2% CaCl2. We have selected two hydrogels: one with a stiffness of 11 +/- 1 kPa, referred to as "low-stiffness hydrogel", formed by 2% alginate and 8% gelatin, and the other with a stiffness of 55 +/- 3 kPa, referred to as "high-stiffness hydrogel", formed by 8% alginate and 12% gelatin. Hydrogel analyses showed that the average swelling rates were 20 +/- 3% for the low-stiffness hydrogels and 35 +/- 2% for the high-stiffness hydrogels. The degradation percentage was 47 +/- 5% and 18 +/- 2% for the low- and high-stiffness hydrogels, respectively. Both hydrogel types showed homogeneous surface shape and protein (Alg-Gel) interaction with CaCl2 as assessed by physicochemical characterization. Cell culture showed good adhesion of the DPSCs to the hydrogels and proliferation. Furthermore, better osteogenic activity, determined by ALP activity and ARS staining, was obtained with high-stiffness hydrogels (8% alginate and 12% gelatin). In summary, this study confirms the possibility of characterizing and optimizing the stiffness of Alg-Gel gel to guide osteogenesis in vitro without altering the other cellular properties of DPSCs.

引用

页数：19