Simultaneous Analysis Method for 27 Endocrine Disrupting Chemicals in Human Urine using UPLC-MS/MS

Endocrine disrupting chemicals (EDCs) are compounds that come from outside the body and disrupt hormone action within the body's endocrine system. Examples include parabens, benzophenones, bisphenols, and phthalates, which are currently used in a wide range of applications. However, continuous exposure to them can have negative effects on glycemic control, reproduction, metabolism, nervous system development, pregnancy, childbirth, and growth. In this study, human samples (urine) were pretreated using liquid-liquid-extraction to determine the exposure level of EDCs and then analyzed effectively and rapidly by UPLC-MS/MS. In this way, the analytical conditions were established and the reliability of the simultaneous analysis method was evaluated through method validation. The results showed that the accuracy ranged from 75.28 to 122.36% and the precision ranged from 2.16 to 22.74%. The analytical method established in this study can be used as a methodology for future studies to evaluate and monitor the exposure of EDCs in human samples.