Evaluation of EUCAST rapid antimicrobial susceptibility test directly from positive blood culture for Pseudomonas aeruginosa

被引：0
|
作者：
Lebreton, Cedric [1 ]
Fournier, Damien [1 ,2 ]
Jeannot, Katy [1 ,2 ,3 ]
机构：
[1] Ctr Hosp Univ Besancon, Lab Bacteriol, Blvd Fleming, F-25000 Besancon, France
[2] Ctr Hosp Univ Besancon, Lab Associe, Ctr Natl Reference Resistance Antibiot, F-25000 Besancon, France
[3] Univ Franche Comte, Chronoenvironm UMR 6249, CNRS, F-25000 Besancon, France
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2024年 / 43卷 / 10期
关键词：
Pseudomonas aeruginosa; RAST; Antibiotic susceptibility testing; Blood culture;
D O I：
10.1007/s10096-024-04895-8
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we evaluated the performance of the EUCAST RAST method on a collection of 154 clinical strains of P. aeruginosa, including strains resistant to ceftazidime and carbapenems. While the test is convenient for routine laboratories, we observed significant rates of VME (ranging from 0.0 to 15.0%) and ME (ranging from 1.3 to 16.3%) after 6 h, particularly for key antibiotics such as ceftazidime, piperacillin/tazobactam, and meropenem. Extending the incubation time to 8 h may improve results (CA ranging from 87.2 to 99%), but caution is required in interpretation due to persistence of VME (ranging from 0.0 to 15.6%) and ME (ranging from 0.0 to 11.7%).
引用
收藏
页码：2061 / 2064
页数：4
相关论文
共 50 条
  • [1] Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) directly from blood culture bottles
    Ying Tong Soo
    Siti Nur Mahirah Bte Waled
    Sally Ng
    Ying Hua Peh
    Ka Lip Chew
    European Journal of Clinical Microbiology & Infectious Diseases, 2020, 39 : 993 - 998
  • [2] Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) directly from blood culture bottles
    Soo, Ying Tong
    Waled, Siti Nur Mahirah Bte
    Ng, Sally
    Peh, Ying Hua
    Chew, Ka Lip
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2020, 39 (05) : 993 - 998
  • [3] Evaluation of EUCAST Direct Rapid Antimicrobial Susceptibility Test Method in Blood Culture Bottles with Positive Signal
    Erdogan, Gizem
    Karakoc, Ayse Esra
    Yucel, Mihriban
    Yagci, Serap
    MIKROBIYOLOJI BULTENI, 2021, 55 (04): : 626 - 634
  • [4] The EUCAST rapid disc diffusion method for antimicrobial susceptibility testing directly from positive blood culture bottles
    Jonasson, Emma
    Matuschek, Erika
    Kahlmeter, Gunnar
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2020, 75 (04) : 968 - 978
  • [5] Implementation of the EUCAST rapid antimicrobial susceptibility test (RAST) directly from positive blood culture bottles without the advanced identification systems
    Taysi, Muhammet Ridvan
    Senturk, Gonul Cicek
    Caliskan, Elif
    Ocal, Duygu
    Miroglu, Gulsah
    Sencan, Irfan
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2022, 77 (04) : 1020 - 1026
  • [6] Usage of 150 mm Mueller - Hinton Agar for the EUCAST rapid antimicrobial susceptibility test (RAST) directly from positive blood culture bottles
    Yildiz, Serap Suzuk
    Sahin, Sevgi
    Tavukcu, Esra
    Mumcuoglu, Ipek
    Hekimoglu, Can Huseyin
    Gureser, Ayse Semra
    Dal, Tuba
    ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 2025, 72 (01) : 43 - 48
  • [7] Multicenter evaluation of rapid antimicrobial susceptibility testing by VITEK®2 directly from positive blood culture
    Paluch, Maxime
    Lleres-Vadeboin, Manon
    Poupet, Helene
    Chanard, Emmanuel
    Wilhelm, Nathalie
    Nadji, Safia
    Prots, Laurence
    Bala, Yohann
    Zambardi, Gilles
    Cattoen, Christian
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2023, 106 (03)
  • [8] Rapid antimicrobial susceptibility of Enterobacteriaceae by disk diffusion directly from blood culture bottles using the EUCAST RAST breakpoints
    Martins, Amanda
    Wink, Priscila
    Pereira, Dariane
    Souza, Andria
    Aquino, Valerio
    Barth, Afonso
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 22 : 637 - 642
  • [9] Validation of EUCAST rapid antimicrobial susceptibility testing directly from positive blood cultures in a non-automated lab setting
    Gregory, Strubbe
    Eveline, Van Honacker
    Stien, Vandendriessche
    Anne-Sophie, Messiaen
    Bruno, Verhasselt
    Jerina, Boelens
    ACTA CLINICA BELGICA, 2024,
  • [10] Evaluation of the EUCAST Rapid Antimicrobial Susceptibility Test for Enterobacterales-Containing Blood Cultures in China
    Shan, Yuzhang
    Hu, Bijie
    Guo, Wei
    Wang, Beili
    Zhou, Chunmei
    Huang, Shenlei
    Li, Na
    JOURNAL OF CLINICAL MICROBIOLOGY, 2022, 60 (04)
12345