Ultra-high-resolution mapping of myelin and g-ratio in a panel of Mbp enhancer-edited mouse strains using microstructural MRI

被引：0

作者：

Grouza, Vladimir ^{[1
,2
]}

Bagheri, Hooman ^{[3
]}

Liu, Hanwen ^{[1
,2
]}

Tuznik, Marius ^{[1
,2
]}

Wu, Zhe ^{[4
]}

Robinson, Nicole ^{[5
,6
]}

Siminovitch, Katherine A. ^{[7
,8
,9
,10
]}

Peterson, Alan C. ^{[2
,11
]}

Rudko, David A. ^{[1
,2
,12
]}

机构：

[1] Montreal Neurol Hosp & Inst, Monnell Brain Imaging Ctr, Montreal, PQ, Canada

[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ, Canada

[3] McGill Univ, Dept Human Genet, Montreal, PQ, Canada

[4] Univ Hlth Network, Krembil Res Inst, Toronto, ON, Canada

[5] McGill Univ, Histol Innovat Platform, Montreal, PQ, Canada

[6] McGill Univ, Morris Goodman Canc Inst, Montreal, PQ, Canada

[7] Univ Toronto, Dept Med, Toronto, ON, Canada

[8] Univ Toronto, Dept Immunol, Toronto, ON, Canada

[9] Mt Sinai Hosp, Lunenfeld Tanenbaum, Toronto, ON, Canada

[10] Toronto Gen Hosp, Res Inst, Toronto, ON, Canada

[11] McGill Univ, Gerald Bronfman Dept Oncol, Montreal, PQ, Canada

[12] McGill Univ, Dept Biomed Engn, Montreal, PQ, Canada

来源：

NEUROIMAGE | 2024年 / 300卷

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Myelin water imaging; Gradient echo MRI; Quantitative MRI; Myelin basic protein; Mouse models; NEURITE ORIENTATION DISPERSION; WATER FRACTION; DIFFUSION PROPERTIES; RELAXATION-TIMES; BRAIN; IMAGE; TEMPERATURE; ACQUISITION; SEPARATION; FIXATION;

D O I：

10.1016/j.neuroimage.2024.120850

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Non-invasive myelin water fraction (MWF) and g-ratio mapping using microstructural MRI have the potential to offer critical insights into brain microstructure and our understanding of neuroplasticity and neuroinflammation. By leveraging a unique panel of variably hypomyelinating mouse strains, we validated a high-resolution, model- free image reconstruction method for whole-brain MWF mapping. Further, by employing a bipolar gradient echo MRI sequence, we achieved high spatial resolution and robust mapping of MWF and g-ratio across the whole mouse brain. Our regional white matter-tract specific analyses demonstrated a graded decrease in MWF in white matter tracts which correlated strongly with myelin basic protein gene (Mbp) Mbp ) mRNA levels. Using these measures, we derived the first sensitive calibrations between MWF and Mbp mRNA in the mouse. Minimal changes in axonal density supported our hypothesis that observed MWF alterations stem from hypomyelination. Overall, our work strongly emphasizes the potential of non-invasive, MRI-derived MWF and g-ratio modeling for both pre- clinical model validation and ultimately translation to humans.

引用

页数：18