Improvement effect of compound Ento-PB on oxazolone-induced ulcerative colitis in rats

被引:0
|
作者
Fan, Zhi [1 ]
Chen, Jinhu [1 ]
Wei, Jia [2 ]
Yang, ZhiBin [1 ,3 ]
Xiao, Huai [1 ,3 ]
Liu, Heng [1 ,3 ]
机构
[1] Dali Univ, Yunnan Prov Key Lab Entomol Biopharmaceut, Dali, Peoples R China
[2] Dali Univ, Natl Local Joint Engn Res Ctr Entomoceut, Dali, Peoples R China
[3] Dali Univ, Engn Res Ctr Dev & Comprehens Utilizat Entomoceut, Dali, Peoples R China
关键词
Colitis; Ulcerative. Oxazolone. Anti-inflammatory Agents; TNBS-INDUCED COLITIS; MICE; DISEASES; EXTRACT; CELLS; MODEL;
D O I
10.1590/acb395524
中图分类号
R61 [外科手术学];
学科分类号
摘要
Purpose: To investigate the impact of the Chinese medicine compound Ento-PB on oxazolone (OXZ)-induced ulcerative colitis (UC) in rats. Methods: UC rats induced by OXZ were treated with Ento-PB. The damage to the colon was assessed using several measures, including the disease activity index (DAI), colon length, colon weight/length ratio, colonic mucosal damage index, and histological score. The levels of interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-13 (IL-13), epidermal growth factor (EGF), inducible nitric oxide synthase, and total nitric oxide synthase (tNOS) in rat serum, as well as the levels of tumor necrosis factor-alpha (TNF-alpha) and myeloperoxidase (MPO) in rat colon tissue, were determined using enzyme-linked immunosorbent assay and conventional kits. Results: After being treated with Ento-PB, the DAI score and macroscopic lesion score of OXZ-induced UC rats were significantly reduced. Ento-PB prevented the shortening of rat colons, reduced the ratio of colon weight to length, and improved colon tissue lesions. Meanwhile, Ento-PB could significantly inhibit the activities of proinflammatory cytokines TNF-alpha, IL-13, and MPO, as well as tNOS and iNOS, while upregulating the expression of anti-inflammatory cytokines IL-4 and IL-10. Moreover, a significant increase in the expression level of EGF was observed in UC rats treated with Ento-PB, indicating that Ento-PB could enhance the repair of damaged intestinal epithelial tissue. Conclusion: Ento-PB demonstrates significant anti-UC activities in OXZ-induced UC rats by regulating the expression levels of inflammatory factors and promoting the repair of colon tissue. This study provides scientific evidence to support the further development of Ento-PB.
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页数:13
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