High-throughput sequencing of archival cerebrospinal fluid specimens defines B-lymphoblastic leukemia clonal composition

被引：0
|
作者：
Foley, Karl [1 ]
Adlowitz, Diana G. [2 ,3 ]
Baker, Cameron [4 ]
Rock, Philip J. [3 ]
Burack, Richard [3 ]
Fries, Carol [2 ,3 ]
机构：
[1] Univ Rochester, Sch Med & Dent, Rochester, NY USA
[2] Univ Rochester, Dept Pediat, Div Pediat Hematol & Oncol, Rochester, NY USA
[3] Univ Rochester, Dept Pathol & Lab Med, Rochester, NY USA
[4] Univ Rochester, Genom Res Ctr, Dept Biostat & Computat Biol, Rochester, NY USA
来源：
PEDIATRIC BLOOD & CANCER | 2024年
关键词：
B-lymphoblastic leukemia; clonal composition; CNS involvement; CSF surveillance; immunoglobulin high-throughput sequencing; pediatric oncology; relapse prediction; response assessment; CENTRAL-NERVOUS-SYSTEM; DISEASE DETECTION; DIAGNOSIS; CHILDREN; RISK;
D O I：
10.1002/pbc.31281
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Detailed characterization of the B-lymphoblastic leukemia (B-ALL) cells which invade the central nervous system (CNS) has been limited by practical challenges. To test whether the clonal composition of the cerebrospinal fluid (CSF) reflects the primary B-ALL tissue, we applied immunoglobulin (Ig) high-throughput sequencing (HTS) of archival CSF cytospin preparations from six patients with morphologically defined CNS involvement. We discovered that most CSF clones are detectable at some timepoint in the primary tissue, but that shifting clonal abundance is prevalent across tissue sites between diagnosis and relapse. Ig HTS of CSF cytospins may improve understanding of sanctuary site dissemination in B-ALL.
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页数：5
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