Doping Control Analysis of Perfluorocarbons in Equine Plasma by Headspace Gas Chromatography-Tandem Mass Spectrometry

被引：0
|
作者：
Kong, Fred K. W. [1 ]
Wong, April S. Y. [1 ]
Cheung, Raymond K. K. [1 ]
Wan, Terence S. M. [1 ]
Ho, Emmie N. M. [1 ]
机构：
[1] Hong Kong Jockey Club, Sha Tin Racecourse, Racing Lab, Sha Tin, Hong Kong, Peoples R China
来源：
DRUG TESTING AND ANALYSIS | 2024年
关键词：
doping in sports; equine plasma; GC/MS/MS; oxygen carriers; perfluorocarbons; OXYGEN CARRIERS; RAT-BLOOD; PERFLUBRON; PERFLUORODECALIN;
D O I：
10.1002/dta.3801
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to control the potential misuse of perfluorocarbons as an oxygen carrier in equine sports, a simple and sensitive method for detecting perfluorocarbons in equine plasma by gas chromatography/tandem mass spectrometry using negative chemical ionization with methane as reagent gas has been developed and fully validated. The method covers seven perfluorocarbons, which are the active components in blood substitute products, and shows good sensitivity and robustness. Limits of detection as low as 0.01 ng/mL could be achieved by the method. To the best of our knowledge, this is the first report of a detection method for the screening of perfluorocarbons in equine biological samples. In order to control the potential misuse of perfluorocarbons as an oxygen carrier in equine sports, a simple and sensitive method for detecting perfluorocarbons in equine plasma by gas chromatography/tandem mass spectrometry has been developed and fully validated. The method covers seven perfluorocarbons, which are the active components in blood substitute products. To the best of our knowledge, this is the first report of a detection method for the screening of perfluorocarbons in equine biological samples.image
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页数：17
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