Metabolism of Fluorinated Topolin Cytokinins in Micropropagated Phalaenopsis amabilis

被引:0
|
作者
Murvanidze, Nino [1 ]
Dolezal, Karel [2 ,3 ,4 ]
Plackova, Lenka [3 ,4 ]
Werbrouck, Stefaan P. O. [1 ]
机构
[1] Univ Ghent, Lab Appl Vitro Plant Biotechnol, Valentin Vaerwyckweg 1, B-9000 Ghent, Belgium
[2] Palacky Univ, Fac Sci, Dept Chem Biol, Slechtitelu 27, Olomouc 78371, Czech Republic
[3] Palacky Univ, Fac Sci, Lab Growth Regulators, Slechtitelu 27, Olomouc 78371, Czech Republic
[4] Inst Expt Bot AS CR, Slechtitelu 27, Olomouc 78371, Czech Republic
关键词
Phalaenopsis; orchid; cytokinin metabolism; in vitro tissue culture; micropropagation; BENZYLADENINE; ZEATIN;
D O I
10.3390/horticulturae10070727
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Fluorinated cytokinins have emerged as promising alternatives to traditional cytokinins in Phalaenopsis plant tissue culture, offering enhanced stability and bioactivity. However, their metabolic fate and impact on endogenous cytokinin profiles remain largely unexplored. This study builds upon previous research to investigate the comparative metabolism of the traditional cytokinin 6-Benzylaminopurine (BA) with the successful alternatives 6-(3-fluorobenzylamino)purine (FmT) and 6-(3-fluorobenzylamino)purine 9-riboside (FmTR). Additionally, this study examines the impact of another crucial factor, the use of ventilated versus closed containers, on metabolic processes. The results revealed the distinct metabolic profiles associated with each treatment, highlighting the complex interplay between exogenous and endogenous cytokinin levels. This study is the first to investigate the effects of these stable, synthetic, and exogenous cytokinins on the naturally occurring cytokinin levels and their metabolites in micropropagated Phalaenopsis. Additionally, we proposed an alternative inactivation pathway involving the conversion of FmTR and BA to pT and pTR. These findings provide valuable insights into the intricate relationship between cytokinin metabolism and plant growth under in vitro conditions.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] FLUORINE CONTAINING TOPOLIN CYTOKININS FOR PHALAENOPSIS AMABILIS (L.) BLUME MICROPROPAGATION
    Murvanidze, Nino
    Dolezal, Karel
    Werbrouck, Stefaan P. O.
    PROPAGATION OF ORNAMENTAL PLANTS, 2019, 19 (02): : 48 - 51
  • [2] New Understanding of Meta-Topolin Riboside Metabolism in Micropropagated Woody Plants
    Grira, Maroua
    Prinsen, Els
    Werbrouck, Stefaan
    PLANTS-BASEL, 2024, 13 (09):
  • [3] Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume
    Saravia-Castillo, Gabriela
    Figueroa, Lourdes Tapia Y.
    Borjas-Ventura, Ricardo
    SCIENTIA AGROPECUARIA, 2022, 13 (01) : 63 - 69
  • [4] Research on the elimination of CyMV and ORSV from Phalaenopsis amabilis
    Wang, R.
    Li, M.
    Yang, P.
    PHYTOPATHOLOGY, 2011, 101 (06) : S187 - S187
  • [5] Shoot development programs in Phalaenopsis amabilis orchid plant
    Semiarti, Endaruz
    Indrianto, Ari
    Suseno, Nilo
    Windiastri, V. Esti
    Isminingsih, Sulastri
    Ishikawa, Takaaki
    Machida, Yasunori
    Machida, Chiyoko
    PLANT AND CELL PHYSIOLOGY, 2007, 48 : S19 - S19
  • [6] SWOT Analysis of Phalaenopsis amabilis Industry in Zhangzhou City
    Xuejiao HE
    Zhicheng YU
    Xiqing ZHUANG
    Plant Diseases and Pests, 2023, (03) : 33 - 36
  • [7] Aromatic cytokinins in micropropagated potato plants
    Baroja-Fernández, E
    Aguirreolea, J
    Martínková, H
    Hanus, J
    Strnad, M
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2002, 40 (03) : 217 - 224
  • [8] In vitro propagation and removal of contaminants in an ornamental orchid (Phalaenopsis amabilis)
    Ghaziani, M. V. Fakouri
    Asa, M. A.
    Kaviani, B.
    Negahdar, N.
    NEW BIOTECHNOLOGY, 2018, 44 : S82 - S83
  • [9] The Effects of Pineapple Peel in Media In Vitro on The Phalaenopsis amabilis Growth
    Budisantoso, Iman
    Kamsinah
    Hikmaturrosyadah, Asna
    SOUTH-EAST ASIAN+ CONFERENCE ON BIODIVERSITY AND BIOTECHNOLOGY 2018, 2020, 593
  • [10] The influence of seed maturation on desiccation tolerance in Phalaenopsis amabilis hybrids
    Schwallier, Rachel
    Bhoopalan, Vanitha
    Blackman, Sheila
    SCIENTIA HORTICULTURAE, 2011, 128 (02) : 136 - 140