Bone marrow mesenchymal stem cells expressing Neat-1, Hotair-1, miR-21, miR-644, and miR-144 subsided cyclophosphamide-induced ovarian insufficiency by remodeling the IGF-1-kisspeptin system, ovarian apoptosis, and angiogenesis

被引：1

作者：

Ahmed, Amany I. ^{[1
]}

Dowidar, Mohamed F. ^{[1
]}

Negm, Asmaa F. ^{[1
]}

Abdellatif, Hussein ^{[2
,3
]}

Alanazi, Asma ^{[4
,5
]}

Alassiri, Mohammed ^{[5
,6
]}

Samy, Walaa ^{[7
]}

Mekawy, Dina Mohamed ^{[8
,9
]}

Abdelghany, Eman M. A. ^{[10
]}

El-Naseery, Nesma I. ^{[11
]}

Ibrahem, Mohamed A. ^{[12
]}

Albadawi, Emad Ali ^{[13
]}

Salah, Wed ^{[14
]}

Eldesoqui, Mamdouh ^{[15
,16
]}

Tirziu, Emil ^{[17
]}

Bucur, Iulia Maria ^{[17
]}

Arisha, Ahmed Hamed ^{[18
,19
]}

Khamis, Tarek ^{[20
]}

机构：

[1] Zagazig Univ, Fac Vet Med, Dept Biochem, Zagazig 44519, Egypt

[2] Sultan Qaboos Univ, Coll Med & Hlth Sci, Dept Human & Clin Anat, Muscat, Oman

[3] Mansoura Univ, Fac Med, Human Anat & Embryol Dept, Mansoura, Egypt

[4] King Saud Bin Abdulaziz Univ Hlth Sci KSAU HS, Coll Sci & Hlth Profess, Riyadh, Saudi Arabia

[5] King Abdullah Int Med Res Ctr, Riyadh, Saudi Arabia

[6] King Saud Bin Abdulaziz Univ Hlth Sci, Coll Sci & Hlth Profess COSHP, Dept Basic Med Sci, Riyadh, Saudi Arabia

[7] Zagazig Univ, Fac Med, Med Biochem Dept, Zagazig 44519, Egypt

[8] Cairo Univ, Fac Med, Med Biochem & Mol Biol Dept, Cairo, Egypt

[9] Zagazig Univ, Fac Med, Human Anat & Embryol Dept, Zagazig 44519, Egypt

[10] Badr Univ Cairo, Fac Med, Dept Med Biochem & Mol Biol, Badr City 11829, Egypt

[11] Zagazig Univ, Fac Vet Med, Dept Histol & Cytol, Zagazig 44519, Egypt

[12] Zagazig Univ, Fac Med, Obstet & Gynecol Dept, Zagazig 44519, Egypt

[13] Taibah Univ, Coll Med, Dept Anat, Medina, Saudi Arabia

[14] Univ Jeddah, Fac Med, Dept Anat, Jeddah, Saudi Arabia

[15] AlMaarefa Univ, Coll Med, Dept Basic Med Sci, POB 71666, Riyadh 11597, Saudi Arabia

[16] Mansoura Univ, Fac Med, Dept Anat & Embryol, Mansoura 35516, Egypt

[17] Univ Life Sci King Mihai I Timisoara ULST, Fac Vet Med, Dept Anim Prod & Vet Publ Hlth, Aradului St 119, Timisoara 300645, Romania

[18] Badr Univ Cairo, Fac Vet Med, Dept Anim Physiol & Biochem, Badr City 11829, Egypt

[19] Zagazig Univ, Fac Vet Med, Dept Physiol & Lab Biotechnol, Zagazig 44511, Egypt

[20] Zagazig Univ, Fac Vet Med, Dept Pharmacol & Lab Biotechnol, Zagazig 44519, Egypt

来源：

JOURNAL OF OVARIAN RESEARCH | 2024年 / 17卷 / 01期

关键词：

lncRNA; microRNAs; Premature ovarian failure; Cyclophosphamide; BM-MSCs; HPG; SMOOTH MUSCLE ACTIN; PROGESTERONE SECRETION; KISSPEPTIN RECEPTOR; FAILURE; CANCER; PROLIFERATION; OOCYTES;

D O I：

10.1186/s13048-024-01498-x

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ovarian insufficiency is one of the common reproductive disorders affecting women with limited therapeutic aids. Mesenchymal stem cells have been investigated in such disorders before yet, the exact mechanism of MSCs in ovarian regeneration regarding their epigenetic regulation remains elusive. The current study is to investigate the role of the bone marrow-derived mesenchymal stem cells (BM-MSCs) lncRNA (Neat-1 and Hotair1) and miRNA (mir-21-5p, mir-144-5p, and mir-664-5p) in mitigating ovarian granulosa cell apoptosis as well as searching BM-MSCs in altering the expression of ovarian and hypothalamic IGF-1 - kisspeptin system in connection to HPG axis in a cyclophosphamide-induced ovarian failure rat model. Sixty mature female Sprague Dawley rats were divided into 3 equal groups; control group, premature ovarian insufficiency (POI) group, and POI + BM-MSCs. POI female rat model was established with cyclophosphamide. The result revealed that BM-MSCs and their conditioned media displayed a significant expression level of Neat-1, Hotair-1, mir-21-5p, mir-144-5p, and mir-664-5p. Moreover, BM-MSCs transplantation in POI rats improves; the ovarian and hypothalamic IGF-1 - kisspeptin, HPG axis, ovarian granulosa cell apoptosis, steroidogenesis, angiogenesis, energy balance, and oxidative stress. BM-MSCs expressed higher levels of antiapoptotic lncRNAs and microRNAs that mitigate ovarian insufficiency.

引用

页数：21

共 1 条

[1] miR-21-loaded bone marrow mesenchymal stem cell-derived exosomes inhibit pyroptosis by targeting MALT1 to repair chemotherapy-induced premature ovarian insufficiency
Lichao Tang
Yutao Yang
Mingxin Yang
Jiaxin Xie
Aiping Zhuo
Yanhong Wu
Mengli Mao
Youhong Zheng
Xiafei Fu
Cell Biology and Toxicology, 41 (1)

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