Protocol for isolating murine kidney tubules and ex vivo cell death assays

被引：1

作者：

Belavgeni, Alexia ^{[1
]}

Maremonti, Francesca ^{[1
]}

Linkermann, Andreas ^{[1
,2
]}

机构：

[1] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Dept Internal Med 3, Div Nephrol, Dresden, Germany

[2] Albert Einstein Coll Med, Dept Med, Div Nephrol, Bronx, NY 10461 USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 02期

关键词：

MICE;

D O I：

10.1016/j.xpro.2024.103005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolating kidney tubules offers insights into their biological function without stroma, vascular cells, and immune system interference. Our murine tubule isolation protocol focuses on ex vivo cell death assays. We describe steps for solution preparation; kidney extraction, decapsulation, and slicing; and tubule isolation. We also outline assays like western blotting, lactate dehydrogenase release assay, and live-cell imaging of vital dyes during experimental acute tubular necrosis. This adaptable protocol allows the generation of outgrown primary tubular cells that maintain the features of tubular cells.

引用

页数：13

共 50 条

[1] Protocol for assessing ex vivo lipolysis of murine adipose tissue
Roy, Debasish
Myers, Julia M.
Tedeschi, Andrea
STAR PROTOCOLS, 2022, 3 (03):
[2] Protocol for isolating and purifying murine liver sinusoidal endothelial cells for in vitro culture and functional assays
Papaioannou, Sophia
See, Jia-Xiang
Baljkas, Tinja
Reiners-Koch, Philipp
Winkler, Manuel
Cerwenka, Adelheid
Stojanovic, Ana
STAR PROTOCOLS, 2025, 6 (01):
[3] Ex vivo regeneration of the murine kidney from human mesenchymal stem cells
Yokoo, T
Kawamura, T
KIDNEY INTERNATIONAL, 2005, 68 (05) : 1967 - 1967
[4] Ex vivo programmed cell death and the prediction of response to chemotherapy
Nagourney R.A.
Current Treatment Options in Oncology, 2006, 7 (2) : 103 - 110
[5] Functional Decline of the Ex Vivo PerfusedHeart is Not Due to Cell Death
Hatami, S.
Qi, X.
Haromy, A.
Aboelnazar, N.
Himmat, S.
White, C. W.
Ondrus, M.
Kinnear, A.
Wu, A.
Buchko, M.
Nagendran, J.
Freed, D. H.
JOURNAL OF HEART AND LUNG TRANSPLANTATION, 2018, 37 (04): : S226 - S226
[6] Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system
Ma, Shengyun
Hernandez, Juan E.
Huang, Wendy Jia Men
STAR PROTOCOLS, 2022, 3 (03):
[7] Identification of receptor ligands by screening phage-display peptide libraries ex vivo on microdissected kidney tubules
Odermatt, A
Audigé, A
Frick, C
Vogt, B
Frey, BM
Frey, FJ
Mazzucchelli, L
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2001, 12 (02): : 308 - 316
[8] Ex-vivo analysis of programmed cell death on fibrolamellar carcinoma
Ajay, Olutani-Grace
Kent, Paul
Nagourney, Robert Alan
Stockwell, Tom
Ellison, Jessica
Swasey, Susanne M.
Tasse, Jordan C.
Schadde, Erik
Humar, Abhinav
Kato, Tom
Yamashiro, Darrell
JOURNAL OF CLINICAL ONCOLOGY, 2023, 41 (16)
[9] Utility of Cell Viability Assays for Use With Ex Vivo Vocal Fold Epithelial Tissue
Erickson-DiRenzo, Elizabeth
Sivasankar, M. Preeti
Thibeault, Susan L.
LARYNGOSCOPE, 2015, 125 (05): : E180 - E185
[10] Technical Considerations in Ex Vivo Human Regulatory T Cell Migration and Suppression Assays
Irekeola, Ahmad Adebayo
Syafirah, Engku Nur E. A. R.
Lazim, Norhafiza Mat
Mohamud, Rohimah
Yean, Chan Yean
Shueb, Rafidah Hanim
CELLS, 2020, 9 (02)

← 1 2 3 4 5 →