Extrusion-Based Bioprinting in a Cost-Effective Bioprinter

被引:0
|
作者
Dharmaraj, Jones Joseph Jebaraj [1 ]
Navasingh, Rajesh Jesudoss Hynes [1 ,2 ]
Krolczyk, Grzegorz [2 ]
Pitchumani, Shenbaga Velu [3 ]
机构
[1] Mepco Schlenk Engn Coll, Dept Mech Engn, Sivakasi 626005, Tamil Nadu, India
[2] Opole Univ Technol, Fac Mech Engn, 76 Proszkowska St, PL-45758 Opole, Poland
[3] Vellore Inst Technol, Sch Mech Engn, Chennai 600127, Tamil Nadu, India
关键词
bioprinting; bioprinter; tissue engineering; syringe pump; DIY bioprinter; extrusion bioprinting; bio-ink; CELL; TISSUES;
D O I
10.3390/machines12080518
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Three-dimensional (3D) bioprinting has emerged as a revolutionary approach in the life sciences, combining multiple disciplines such as computer engineering, materials science, robotics, and biomedical engineering. This innovative technology enables the production of cellular constructs using bio-inks, and differs from conventional 3D printing by incorporating living cells. The present work addresses the conversion of a commercial thermoplastic 3D printer into a low-cost bioprinter. The modification addresses the challenges of the high cost of commercial bioprinters, limited adaptability, and specialized personnel requirements. This modification uses an extrusion-based bioprinting method that is particularly popular in research due to its viscosity tolerance and versatility. The individual steps, including replacing the extruder with a syringe pump, rebuilding the electronic motherboard, and configuring the firmware, are explained in detail. The work aims at providing access to bioprinting technology so that laboratories with modest resources can take advantage of the immense potential of this technology. This modification resulted in improved resolution, allowing submicron movements, which is comparable to some of the commercially available bioprinters. The accuracy of the modified printer was validated using hydrogel bioprinting tests, suggesting that it is suitable for broader applications in regenerative medicine.
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页数:18
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