Chemical Profiling and Cytotoxicity Activity of Essential Oil from Artemisia Maritima Collected through Four Different Wild Locations of Lahaul Valley in Indian Western Himalaya

This research leads to the analysis of quality parameters and chemical profiles of Artemisia maritima essential oils (EOs) extracted from four different wild areas viz: Darcha, Jispa, Gemur and Tandi of Lahaul valley of Indian western Himalaya in comparison to a market sample. The EOs content was found higher in Darcha (0.54 %) and Jispa (0.48 %). The quality parameters such as color, odor, density, refractive index and optical rotations were found comparable with the market sample. Further, volatile chemo-profile of EOs was generated using GC/GC-MS. A total of 34 metabolites were identified in five samples (n=3) that account around 87.3-95.1 % of the total oils. These EOs majorly contained monoterpene hydrocarbons (3.0-68.5 %) and oxygenated monoterpenes (26.6-85.0 %) with beta-myrcene (9.8-20.6 %), alpha-terpinene (0.7-17.3 %), beta-phellandrene (1.5-22.4 %), 1,8-cineole (3.0-50.8 %), terpinen-4-ol (2.5-7.2 %), ascaridole (11.5 %), bornyl acetate (5.1-14.2 %), trans-sabinyl acetate (0.8-49.6) and trans-ascaridole (7.9 %). Moreover, cytotoxicity study of EOs against CaCo2 and HT-29 human colon carcinoma cell lines unveiled significant anticancer potential of A. maritima. AMD (82.87 +/- 0.39 %) AMG (61.17 +/- 1.45 %) and AMM (73.37 +/- 2.1 %) showed comparable activity at 600 mu g/ml (72 hr) in comparisons to vinblastine (20 mu M). A. maritima is less explored as compared to other species and could be a new potential source for EOs and anticancer candidate.