Identification and Analysis of WRKY Transcription Factors in Response to Cowpea Fusarium Wilt in Cowpea

被引：0

作者：

Hao, Yali ^{[1
,2
]}

Liu, Rui ^{[2
]}

Mao, Zhenchuan ^{[2
]}

Yang, Qihong ^{[2
]}

Zheng, Shijie ^{[2
]}

Lu, Xiaofei ^{[3
]}

Yang, Yuhong ^{[2
]}

Xie, Bingyan ^{[2
]}

Zhao, Jianlong ^{[2
]}

Li, Yan ^{[2
]}

Chen, Guohua ^{[2
]}

Ling, Jian ^{[1
,2
]}

机构：

[1] Shanxi Agr Univ, Coll Hort, Jinzhong 030810, Peoples R China

[2] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

[3] Inst Zhongnong Tuba, Beijing 100081, Peoples R China

来源：

PLANTS-BASEL | 2024年 / 13卷 / 16期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

cowpea; cowpea Fusarium wilt; WRKY; Fusarium oxyporum; transcription regulation; expression profiling; DNA-BINDING; GENE SUPERFAMILY; FACTOR FAMILY; ARABIDOPSIS; EXPRESSION; PROTEINS; ACID; RESISTANCE; STRESS;

D O I：

10.3390/plants13162273

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In plants, WRKY transcription factors play a crucial role in plant growth, development, and response to abiotic and biotic stress. Cowpea (Vigna unguiculata) is an important legume crop. However, cowpea Fusarium wilt (CFW), caused by Fusarium oxysporum f. sp. tracheiphilum (Fot), poses a serious threat to its production. In this study, we systematically identified members of the cowpea WRKY (VuWRKY) gene family and analyzed their expression patterns under CFW stress. A total of 91 WRKY transcription factors were identified in the cowpea genome. Phylogenetic and synteny analyses indicated that the expansion of VuWRKY genes in cowpea is primarily due to recent duplication events. Transcriptome analysis of cowpea inoculated with Fo revealed 31 differentially expressed VuWRKY genes, underscoring their role in the response to CFW infection. Four differentially expressed WRKY genes were selected for validation. Subcellular localization and Western blot assays showed their nuclear localization and normal expression in N. benthamiana. Additionally, yeast one-hybrid assays demonstrated that VuWRKY2 can bind to the promoter region of the Catalase (CAT) gene, indicating its potential role in transcriptional regulation. This study establishes a foundation for further exploration of the role and regulatory mechanisms of VuWRKY genes in response to CFW stress.

引用

页数：18