SWATH-proteomics reveals Mathurameha, a traditional anti-diabetic herbal formula, attenuates high glucose-induced endothelial dysfunction through the EGF/NO/IL-1β regulatory axis

被引：1

作者：

Aluksanasuwan, Siripat ^{[1
,2
,8
]}

Somsuan, Keerakarn ^{[1
,2
]}

Chiangjong, Wararat ^{[3
]}

Rongjumnong, Artitaya ^{[2
]}

Jaidee, Wuttichai ^{[4
]}

Rujanapun, Narawadee ^{[4
]}

Chutipongtanate, Somchai ^{[5
]}

Laphookhieo, Surat ^{[4
,6
]}

Charoensup, Rawiwan ^{[4
,7
]}

机构：

[1] Mae Fah Luang Univ, Sch Med, Chiang Rai 57100, Thailand

[2] Mae Fah Luang Univ, Canc & Immunol Res Unit CIRU, Chiang Rai 57100, Thailand

[3] Mahidol Univ, Pediat Translat Res Unit, Dept Pediat, Fac Med Ramathibodi Hosp, Bangkok 10400, Thailand

[4] Mae Fah Luang Univ, Med Plants Innovat Ctr, Chiang Rai 57100, Thailand

[5] Univ Cincinnati, Coll Med, Dept Environm & Publ Hlth Sci, Cincinnati, OH 45267 USA

[6] Mae Fah Luang Univ, Ctr Chem Innovat Sustainabil CIS, Sch Sci, Chiang Rai 57100, Thailand

[7] Mae Fah Luang Univ, Sch Integrat Med, Chiang Rai 57100, Thailand

[8] Mae Fah Luang Univ, Sch Med, 333 Moo 1, Muang 57100, Chiang Rai, Thailand

来源：

JOURNAL OF PROTEOMICS | 2024年 / 306卷

关键词：

Diabetes; Endothelial cells; High glucose; Epidermal growth factor; Mathurameha; GROWTH-FACTOR RECEPTOR; DIABETES-MELLITUS; OXIDATIVE STRESS; NITRIC-OXIDE; EXTRACTS; KINASE; CELLS; ENRICHMENT; EXPRESSION; PROTECTS;

D O I：

10.1016/j.jprot.2024.105263

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mathurameha is a traditional Thai herbal formula with a clinically proven effect of blood sugar reduction in patients with diabetes mellitus, but its anti-diabetic complication potential is largely unknown. This study aimed to elucidate the effects of Mathurameha and its underlying mechanisms against high glucose-induced endothelial dysfunction in human endothelial EA.hy926 cells. After confirming no cytotoxic effects, the cells were treated with normal glucose (NG), high glucose (HG), or high glucose plus Mathurameha (HG + M) for 24 h. A quantitative label-free proteomic analysis using the sequential window acquisition of all theoretical mass spectra (SWATH-MS) approach identified 24 differentially altered proteins among the three groups: 7 between HG and NG, 9 between HG + M and NG, and 13 between HG + M and HG. Bioinformatic analyses suggested a potential anti-diabetic action through the epidermal growth factor (EGF) pathway. Subsequent functional validations demonstrated that Mathurameha reduced the EGF secretion and the intracellular reactive oxygen species (ROS) level in high glucose-treated cells. Mathurameha also exhibited a stimulatory effect on nitric oxide (NO) production while significantly reducing the secretion of endothelin-1 (ET-1) and interleukin-1 beta (IL-1 beta) in high glucose-treated cells. In conclusion, our findings demonstrated that Mathurameha attenuated high glucoseinduced endothelial dysfunction through the EGF/NO/IL-1 beta regulatory axis. Significance: This study reveals the potential of Mathurameha, a traditional Thai herbal formula, in mitigating high glucose-induced endothelial dysfunction, a common complication in diabetes mellitus. Using proteomics and bioinformatic analyses followed by functional validations, the present study highlights the protective effects of Mathurameha through the EGF/NO/IL-1 beta regulatory axis. These findings support its potential use as a therapeutic intervention for diabetic vascular complications and provide valuable information for developing more effective anti-diabetic drugs.

引用

页数：11