Two HbpA-like proteins HbpA1 and HbpA2 from Actinobacillus pleuropneumoniae protect bacteria from sulfur source limitation, oxidative and cold stresses, but not essential to virulence

被引：0

作者：

Zhang, Miao ^{[1
]}

Li, Zhuo ^{[1
]}

Hu, Hanwen ^{[1
]}

Liu, Jinlin ^{[1
]}

Qi, Chao ^{[1
]}

机构：

[1] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China

来源：

GENE | 2024年 / 931卷

关键词：

Actinobacillus pleuropneumoniae; HbpA1; HbpA2; GSH utilization; Stress tolerance; Virulence; GLUTATHIONE IMPORT; IRON; ACQUISITION;

D O I：

10.1016/j.gene.2024.148875

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Porcine pleuropneumonia is one of the respiratory diseases that pigs are susceptible to Actinobacillus pleuropneumoniae (A. pleuropneumoniae), poses a great threat to the global pig industry. Glutathione (GSH) is an important sulfur source, cellular antioxidant and virulence determinant of many pathogenic bacteria. In this study, roles of two HbpA-like proteins HbpA1 and HbpA2 of A. pleuropneumoniae were analyzed. A. pleuropneumoniae mutants without HbpA2 were basically unable to grow in chemically defined medium (CDM) with GSH as the sole sulfur source and had significantly reduced oxidative tolerance; whereas mutation in hbpA1 led to reduced survival under low-temperature environments. Neither HbpA1 nor HbpA2 affects utilization of heme. These two HbpA-like proteins are not associated with the virulence of A. pleuropneumoniae. Our results reveal the correlation of A. pleuropneumoniae HbpA1 and HbpA2 in GSH utilization, highlight the roles of HbpA1 in the cold stress resistance and HbpA2 in the anti-oxidative response. GSH limitation is not a way to attenuate colonization and pathogenicity of A. pleuropneumoniae.

引用

页数：8