A hybrid genome assembly of the endangered aye-aye (Daubentonia madagascariensis)

被引:3
|
作者
Versoza, Cyril J. [1 ]
Pfeifer, Susanne P. [1 ]
机构
[1] Arizona State Univ, Ctr Evolut & Med, Sch Life Sci, Tempe, AZ 85281 USA
来源
G3-GENES GENOMES GENETICS | 2024年 / 14卷 / 10期
关键词
aye-aye; Daubentonia madagascariensis; Daubentoniidae; strepsirrhine; primate; hybrid assembly; DE-NOVO IDENTIFICATION; SEQUENCE; FAMILIES; COVERAGE; LEMUR;
D O I
10.1093/g3journal/jkae185
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The aye-aye (Daubentonia madagascariensis) is the only extant member of the Daubentoniidae primate family. Although several reference genomes exist for this endangered strepsirrhine primate, the predominant usage of short-read sequencing has resulted in limited assembly contiguity and completeness, and no protein-coding gene annotations have yet been released. Here, we present a novel, fully annotated, chromosome-level hybrid de novo assembly for the species based on a combination of Oxford Nanopore Technologies long reads and Illumina short reads and scaffolded using genome-wide chromatin interaction data-a community resource that will improve future conservation efforts as well as primate comparative analyses.
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页数:6
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