Total flavonoids of litchi Seed alleviates schistosomiasis liver fibrosis in mice by suppressing hepatic stellate cells activation and modulating the gut microbiomes

被引:0
|
作者
Li, Qing [1 ,2 ,3 ]
Wang, Jilong [4 ]
Lv, Jiahui [4 ]
Liu, Dengyu [4 ]
Xiao, Suyu [4 ]
Mo, Jingquan [5 ]
Lu, Zuochao [4 ]
Qiu, Ran [5 ]
Li, Caiqi [5 ]
Tang, Lili [4 ]
He, Shanshan [4 ]
Tang, Zeli [1 ,2 ,3 ]
Cheng, Qiuchen [3 ,6 ]
Zhan, Tingzheng [2 ,3 ,4 ]
机构
[1] Guangxi Med Univ, Dept Cell Biol & Genet, Nanning, Guangxi, Peoples R China
[2] Guangxi Med Univ, Key Lab Longev & Aging Related Dis, Chinese Minist Educ, Nanning, Guangxi, Peoples R China
[3] Guangxi Med Univ, Educ Dept Guangxi Zhuang Autonomous Reg, Key Lab Basic Res Reg Dis, Nanning, Guangxi, Peoples R China
[4] Guangxi Med Univ, Dept Parasitol, Nanning, Guangxi, Peoples R China
[5] Guangxi Med Univ, Sch Preclin Med, Nanning, Peoples R China
[6] Guangxi Acad Med Sci, Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Gastroenterol, Nanning, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Total Flavonoids of Litchi Seed; Schistosoma japonicum; Liver fibrosis; Hepatic stellate cells (HSCs); Gut microbiomes; DENDRITIC CELLS; T-CELLS; PATHOGENESIS; ALCOHOL; IMMUNOBIOLOGY; POLARIZATION; METABOLITES; INDUCTION; INFECTION; DISTINCT;
D O I
10.1016/j.biopha.2024.117240
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Infection with Schistosoma japonicum (S. japonicum) is an important zoonotic parasitic disease that causes liver fibrosis in both human and domestic animals. The activation of hepatic stellate cells (HSCs) is a crucial phase in the development of liver fibrosis, and inhibiting their activation can alleviate this progression. Total flavonoids of litchi seed (TFL) is a naturally extracted drug, and modern pharmacological studies have shown its anti-fibrotic and liver-protective effects. However, the role of TFL in schistosomiasis liver fibrosis is still unclear. This study investigated the therapeutic effects of TFL on liver fibrosis in S. japonicum infected mice and explored its potential mechanisms. Animal study results showed that TFL significantly reduced the levels of Interleukin-1 beta (IL1 beta), Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-4 (IL-4), and Interleukin-6 (IL-6) in the serum of S. japonicum infected mice. TFL reduced the spleen index of mice and markedly improved the pathological changes in liver tissues induced by S. japonicum infection, decreasing the expression of alpha-smooth muscle actin (alpha-SMA), Collagen I and Collagen III protein in liver tissues. In vitro studies indicated that TFL also inhibited the activation of HCSs induced by Transforming Growth Factor-beta 1 (TGF-beta 1) and reduced the levels of alpha-SMA. Gut microbes metagenomics study revealed that the composition, abundance, and functions of the mice gut microbiomes changed significantly after S. japonicum infection, and TLF treatment reversed these changes. Therefore, our study indicated that TFL alleviated granulomatous lesions and improved S. japonicum induced liver fibrosis in mice by inhibiting the activation of HSCs and by improving the gut microbiomes.
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页数:14
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