Eco-friendly synthesis of Co3O4 nanoparticles using Millettia pinnata towards increased anti-oxidant, anti-biofilm and cytocompatibility properties against biofilm producing bacteria and human pulmonary alveolar basal cells

被引：0

作者：

Margoni, Mudaliar Mahesh ^{[1
]}

Rajivgandhi, Govindan ^{[2
]}

Gnanasekaran, Chackaravarthi ^{[3
]}

Chelliah, Chenthis Kanisha ^{[4
]}

Basha, S. Sathik ^{[4
]}

Ramachandran, Govindan ^{[2
]}

Maruthupandy, Muthuchamy ^{[5
]}

Dhanasekar, M. ^{[6
]}

Quero, Franck ^{[2
]}

Akbari-Fakhrabadi, Ali ^{[1
]}

Bhaviripudi, Vijayabhaskara Rao ^{[7
]}

Kadaikunnan, Shine ^{[8
]}

Almanaa, Taghreed N. ^{[8
]}

机构：

[1] Univ Chile, Fac Phys Sci & Math, Dept Mech Engn, Adv Mat Lab AmLab, Ave Beauchef-851, Santiago, Chile

[2] Univ Chile, Fac Ciencias Fis & Matemat, Dept Ingn Quim Biotecnol & Mat, Lab Nanocelulosa & Biomat, Ave Beauchef 851, Santiago 8370456, Chile

[3] Bharathidasan Univ, Dept Marine Sci, Tiruchirappalli 620024, Tamil Nadu, India

[4] BS Abdur Rahman Crescent Inst Sci & Technol, Dept Phys, Vandalur 600048, Tamil Nadu, India

[5] Dong A Univ, Grad Sch, Dept Hlth Sci, Lab Toxicol, 37 Nakdong Dearo 550 Beon Gil, Busan 49315, South Korea

[6] SRM Inst Sci & Technol, Dept Phys, Ramapuram Campus, Chennai 600089, Tamil Nadu, India

[7] Univ Tecnol Metropolitana, Dept Fis, Fac Ciencias Nat Matemat & Medio Ambiente, Santiago 7800002, Chile

[8] King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2024年 / 100卷

关键词：

Nanoparticles; Plant extract; Anti-oxidant; Anti-Biofilm activity; Membrane integrity; Cytotoxicity; EXTRACT; NANOSTRUCTURES;

D O I：

10.1016/j.jddst.2024.106124

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Biofilm architecture is an emerging threat worldwide due to enhanced antibiotic resistance against existing antibiotics, thus heightening the need to discover the alternative therapies that can eradicate the biofilm colonization. Current study highlights the eco-friendly, non-toxic nature of Co3O4 nanoparticles (NPs) using Millettia pinnata (M. pinnata) leaves extract and their application in anti-oxidants, anti-bacterial, anti-biofilm and cytocompatibilities. The GC-MS and FT-IR spectral data suggested that phytochemical derivatives of M. pinnata leaf extract simultaneously contributed in bio-reduction process and capping agent of Co3O4 NPs, which might be reason for anti-oxidant activities. Further, XRD analysis confirms the polycrystalline behaviour of Co3O4 NPs having hexagonal crystal system. FESEM, HRTEM, EDAX and elemental mapping revealed the formation of agglomerated granular shape along with nanorods on the surface of Co3O4 NPs, which confirms the presence of carbon and oxygen. Additionally, the characteristic band and thermal decomposition of Raman and TGA spectral data confirmed the molecular structure of Co3O4 NPs. Subsequently, both the M. pinnata leaves extract and Co3O4 NPs were found to be excellent source of antioxidants and produced significant DPPH and hydroxyl scavenging activities. Furthermore, the sub-biofilm inhibitory concentration of biosynthesized Co3O4 NPs reduced the biofilm growth in S. aureus and K. pneumoniae by crystal violet assay at increasing concentration. In addition, the membrane integrity of biofilm damages and architecture destruction was evidently proved due to the biosynthesized Co3O4 NPs by confocal laser scanning electron microscope and scanning electron microscope observation. Finally, the cytotoxicity experiment results indicated, biosynthesized Co3O4 NPs has no toxicity against human pulmonary alveolar epithelial cells at maximum concentration. Accordingly, findings of this study supported the utility of safety and efficiency of biosynthesized Co3O4 NPs against biofilm producing bacteria, and deserve as promising future drug discovery target for various bacterial infections.

引用

页数：15