Protocol Protocol for transplantation of cells derived from human midbrain organoids into a Parkinson's disease mouse model to restore motor function

被引：0
|
作者：
Fu, Chong-Lei [2 ]
Jiang, Xi [1 ]
Dong, Bo-Cheng [1 ]
Li, Dan [3 ]
She, Xin-Yu [1 ]
Yao, Jun [1 ]
机构：
[1] Tsinghua Univ, Tsinghua Peking Ctr Life Sci, State Key Lab Membrane Biol, IDG McGovern,Inst Brain Res,Sch Life Sci, Beijing 100084, Peoples R China
[2] Shandong First Med Univ & Shandong Acad Med Sci, Shandong Inst Brain Sci & Brain Inspired Res, Jinan 250117, Peoples R China
[3] Peking Univ Third Hosp, Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 03期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.xpro.2024.103251
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Midbrain organoids provide an innovative cellular source for transplantation therapies of neurodegenerative diseases. Here, we present a protocol for midbrain organoid-derived cell transplantation into a Parkinson's disease mouse model. We describe steps for midbrain organoid generation, single-cell suspension preparation, and cell transplantation. This approach is valuable for studying the efficacy of midbrain organoids as a potential cellular source for restoring motor function. For complete details on the use and execution of this protocol, please refer to Fu et al.1 1
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页数：13
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