Kinesthetic orientation refers to the human body's perception of the position and direction of its own body parts relative to space. It is realized through the information provided by the human body's motion sensory organs to perceive its position and direction in space and the relative position between body parts, so it has a very important role. Firstly, this paper analyzes the right and left hands to get the conclusion that the dominant hand is stronger than the weak hand, and then eight factors affecting the orientation of kinaesthesia are selected and analyzed and studied, including age, gender, arm pressure, heart rate, ambient chromatic aberration, ambient music, muscle memory, and body posture. Through the multiple regression analysis, correlation coefficient test, and gray correlation analysis of the above factors, it is concluded that muscle memory is a relatively important influencing factor, and based on this, relevant theoretical suggestions are put forward. Then this paper introduces the concept of kinesthetic orientation muscle memory index. to measure the level of muscle memory ability in the kinesthetic orientation test, which will be defined as 1-Delta theta/0.4 theta (where Delta theta represents the error of the rotation angle, represents the target angle). Next, based on the definition of the kinesthetic orientation muscle memory index to further carry out research on the kinesthetic orientation muscle memory, the kinesthetic orientation muscle memory forgetting law to explore and study, and get the results of the time as the We obtained the kinesthetic orientation muscle memory forgetting law curve under different training conditions with time as the horizontal coordinate and the kinesthetic orientation muscle memory index as the vertical coordinate, and found the lawof the fastest decline point of muscle memory, which can be used as a guide and reference to guide the patients to recover their kinesthetic awareness quickly or the athletes to improve their kinesthetic orientation perception.