Genomic characterization of carbapenemase-producing Enterobacterales from Dhaka food markets unveils the spread of high-risk antimicrobial-resistant clones and plasmids co-carrying blaNDM and mcr-1.1

被引：0

作者：

Nisa, Tania Tabassum ^{[1
]}

Sugawara, Yo ^{[2
,3
]}

Hamaguchi, Shigeto ^{[4
,5
,6
]}

Takeuchi, Dan ^{[3
]}

Abe, Ryuichiro ^{[3
]}

Kuroda, Eisuke ^{[6
,7
]}

Morita, Masatomo ^{[8
]}

Zuo, Hui ^{[2
]}

Ueda, Akiko ^{[9
]}

Nishi, Isao ^{[9
]}

Hossain, Nowrin ^{[10
]}

Hasan, Md Mahmudul ^{[10
]}

Siddiqee, Mahbubul H. ^{[10
]}

Nakatani, Daisaku ^{[1
]}

Nakata, Ken ^{[1
]}

Akeda, Yukihiro ^{[3
,8
]}

机构：

[1] Osaka Univ, Grad Sch Med, Global & Innovat Med, Osaka, Japan

[2] Natl Inst Infect Dis, Antimicrobial Resistance Res Ctr, Tokyo, Japan

[3] Osaka Univ, Res Inst Microbial Dis, Osaka, Japan

[4] Osaka Univ Hosp, Div Infect Control & Prevent, Osaka, Japan

[5] Osaka Univ, Grad Sch Med, Dept Transformat Anal Human Specimen, Osaka, Japan

[6] Osaka Univ, Ctr Infect Dis Educ & Res CiDER, Div Fostering Required Med Human Resources, Osaka, Japan

[7] Osaka Univ, Grad Sch Med, Dept Transformat Infect Control Dev Studies, Osaka, Japan

[8] Natl Inst Infect Dis, Dept Bacteriol 1, Tokyo, Japan

[9] Osaka Univ Hosp, Lab Clin Invest, Osaka, Japan

[10] BRAC Univ, Dept Math & Nat Sci MNS, Mol & Environm Microbiol MEM Lab, Dhaka, Bangladesh

来源：

JAC-ANTIMICROBIAL RESISTANCE | 2024年 / 6卷 / 04期

关键词：

ESCHERICHIA-COLI; BETA-LACTAMASE; EVOLUTION;

D O I：

10.1093/jacamr/dlae124

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: The transmission of carbapenemase-producing Enterobacterales (CPE) in the external environment, especially through food, presents a significant public health risk. Objectives To investigate the prevalence and genetic characteristics of CPE in food markets of Dhaka, Bangladesh, using WGS. Methods: CPE isolates were obtained from different food and water samples collected from food markets in the southern part of Dhaka, Bangladesh. The isolates subsequently underwent molecular typing, WGS employing both short- and long-read sequencers, and plasmid analysis. Results: This study unveiled an extensive spread of CPE, with no significant difference in contamination rates observed in samples (N = 136), including meat (n = 8), fish (n = 5), vegetables (n = 36) or various food-washed water (n = 65) from markets near hospitals or residential areas. Thirty-eight Enterobacterales from 33 samples carried carbapenemase genes (bla(NDM-1, -4, -7), bla(KPC-2), bla(OXA-181) or bla(IMI-1)). Among these, the high-risk Escherichia coli ST410 clone was the most prevalent and distributed across various locations. Furthermore, the identification of IncHI2 plasmids co-harbouring resistance genes like bla(NDM-5) and mcr-1.1, without discernible epidemiological connections, is a unique finding, suggesting their widespread dissemination. Conclusions: The analysis unveils a dynamic landscape of CPE dissemination in food markets, underscored by the proliferation of novel IncHI2 hybrid plasmids carrying both colistin- and carbapenem-resistance genes. This illuminates the ever-evolving landscape of antimicrobial resistance in Dhaka, urging us to confront its emergent challenges.

引用

页数：11