Visual Tracking of Hydrogen Sulfide: Application of a Novel Lysosome-Targeted Fluorescent Probe for Bioimaging and Food Safety Assessment

被引:0
|
作者
Liu, Likun [1 ]
Liu, Yitong [2 ]
Ren, Haoqing [2 ]
Hou, Peng [2 ]
Wang, Haijun [2 ]
Sun, Jingwen [2 ]
Liu, Lei [2 ]
He, Chuan [2 ]
Chen, Song [2 ]
机构
[1] Qiqihar Med Univ, Res Inst Med & Pharm, Qiqihar 161006, Peoples R China
[2] Qiqihar Med Univ, Coll Pharm, Qiqihar 161006, Peoples R China
来源
MOLECULES | 2024年 / 29卷 / 16期
关键词
hydrogen sulfide; fluorescence probe; turn-on response; bioimaging; foodstuff; DESIGN; H2S;
D O I
10.3390/molecules29163906
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The equilibrium state of hydrogen sulfide (H2S), a gaseous signaling molecule produced by lysosomal metabolites, in vivo is crucial for cellular function. Abnormal fluctuations in H2S concentration can interfere with the normal function of lysosomes, which has been closely linked to the pathogenesis of a variety of diseases. In view of this, a novel fluorescent probe Lyso-DPP based on 1,3,5-triarylpyrazolines was developed for the precise detection of H2S in lysosomes by using the hydrophilic morpholine moiety as a lysosomal targeting unit, and 2,4-dinitroanisole as a fluorescence-quenching and H2S-responsive unit. The probe cleverly combines the advantages of simple synthesis, sensitive blue fluorescence turn-on with a limit of detection, LOD, of 97.3 nM, good stability, and fast response time (10 min), which makes Lyso-DPP successful in portable monitoring of meat freshness in the form of test strips. Moreover, the excellent biocompatibility and precise targeting capability of the probe Lyso-DPP make it perform well in the monitoring of H2S in lysosomes, living cells, and zebrafish. This work not only provides new technical tools for food quality control but also paves up new ideas for early diagnosis and treatment of H2S-related diseases.
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页数:14
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