Non-sterile cultivation of Yarrowia lipolytica in fed-batch mode for the production of lipids and biomass

被引:0
|
作者
Chitnis, Atith V. [1 ]
Dhoble, Abhishek S. [1 ]
机构
[1] Indian Inst Technol BHU, Sch Biochem Engn, Varanasi 221005, Uttar Pradesh, India
关键词
flow cytometry; lipid accumulation; nonsterile; oleaginous yeasts; Yarrowia lipolytica; VOLATILE FATTY-ACIDS; SINGLE-CELL OIL; ACETIC-ACID; CONVERSION; FERMENTATION; ACCUMULATION; BIODIESEL; GROWTH; WASTE;
D O I
10.1002/btpr.3498
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A reduction in the cost of production and energy requirement is necessary for developing sustainable commercial bioprocesses. Bypassing sterilization, which is an energy and cost-intensive part of bioprocesses could be a way to achieve this. In this study, nonsterile cultivation of Yarrowia lipolytica was done on a synthetic medium containing acetic acid as the sole carbon source using two different strategies in the fed-batch mode. The contamination percentages throughout the process were measured using flow cytometry and complemented using brightfield microscopy. Maximum biomass and lipid yields of 0.57 (g biomass/g substrate) and 0.17 (g lipids/g substrate), respectively, and maximum biomass and lipid productivities of 0.085 and 0.023 g/L/h, respectively, were obtained in different fed-batch strategies. Feeding at the point of stationary phase resulted in better biomass yield and productivity with less than 2% contamination till 48 h. Feeding to maintain a minimum acetic level resulted in better lipid yield and productivity with less than 2% contamination during the complete process. The results of this study demonstrate the potential for cultivating Y. lipolytica in nonsterile conditions and monitoring the contamination throughout the process using flow cytometry.
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页数:15
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